A simple and rapid approach to prepare Sindbis and West Nile viral RNA controls for differentiation between positive samples and laboratory contamination.
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DOI: 10.1016/j.jviromet.2020.113822
Abstract: Reverse transcription-polymerase chain reaction (RT-PCR) is frequently used for surveillance and diagnosis of arboviruses and emerging viruses. A disadvantage of RT-PCR assays, especially nested assays, is the potential for false-positive results caused by laboratory contamination… read more here.
Keywords: positive control; positive samples; contamination; laboratory contamination ... See more keywords