Holin‐assisted bacterial recombinant protein export
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28179
Abstract: A simple generic method for enhancing extracellular protein yields in engineered bacteria is still lacking. Here, we demonstrated that phage‐encoded holin can be used to export proteins to the extracellular medium in both Gram‐negative Escherichia… read more here.
Keywords: hol380; holin assisted; holin; lactis ... See more keywords
Immunoregulatory effects of Lactococcus lactis‐derived extracellular vesicles in allergic asthma
Sign Up to like & getrecommendations! Published in 2022 at "Clinical and Translational Allergy"
DOI: 10.1002/clt2.12138
Abstract: Abstract Background Probiotics have been shown to prevent various allergic diseases by producing extracellular vesicles (EVs). However, the role of EVs in allergic asthma has not yet been completely determined. Methods Gut microbial composition, mainly… read more here.
Keywords: extracellular vesicles; allergic asthma; lactis; mice ... See more keywords
Enhancing the biosynthesis of nicotinamide mononucleotide in Lactococcus lactis by heterologous expression of FtnadE.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the science of food and agriculture"
DOI: 10.1002/jsfa.12253
Abstract: BACKGROUND Nicotinamide mononucleotide (NMN), a key intermediate of nicotinamide adenine dinucleotide, plays an important in anti-aging and disease. Lactococcus lactis, an important probiotic lactic acid bacteria (LAB), has shown great potential for the biosynthesis of… read more here.
Keywords: nicotinamide mononucleotide; biosynthesis; heterologous expression; lactis ... See more keywords
Evaluation of corn steep liquor as fermentation media for recombinant Lactococcus lactis producing antifreeze proteins.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of the science of food and agriculture"
DOI: 10.1002/jsfa.12461
Abstract: BACKGROUND Corn processing byproducts corn steep liquor (CSL), and thin stillage (TS) were evaluated as growth media for recombinant Lactococcus lactis modified to produce antifreeze proteins (AFPs) that could have important food and non-food applications.… read more here.
Keywords: corn; corn steep; growth; food ... See more keywords
The Lactococcus lactis KF147 nonribosomal peptide synthetase/polyketide synthase system confers resistance to oxidative stress during growth on plant leaf tissue lysate
Sign Up to like & getrecommendations! Published in 2018 at "MicrobiologyOpen"
DOI: 10.1002/mbo3.531
Abstract: Strains of Lactococcus lactis isolated from plant tissues possess adaptations that support their survival and growth in plant‐associated microbial habitats. We previously demonstrated that genes coding for a hybrid nonribosomal peptide synthetase/polyketide synthase (NRPS/PKS) system… read more here.
Keywords: lactis; nrps pks; system; plant ... See more keywords
Bacteriocinogenic Lactococcus lactis subsp. lactis 3MT isolated from freshwater Nile Tilapia: isolation, safety traits, bacteriocin characterisation, and application for biopreservation in fish pâté
Sign Up to like & getrecommendations! Published in 2019 at "Archives of Microbiology"
DOI: 10.1007/s00203-019-01690-4
Abstract: This work was aimed to screen bacteriocin-producing LAB from freshwater fish, select a prominent strain and evaluate its safety, characterise the bacteriocin produced, and evaluate its potential to be used as biopreservatives. Isolate 3MT showed… read more here.
Keywords: lactis; freshwater; safety; subsp lactis ... See more keywords
Analysis of the cell wall binding domain in bacteriocin-like lysin LysL from Lactococcus lactis LAC460
Sign Up to like & getrecommendations! Published in 2024 at "Archives of Microbiology"
DOI: 10.1007/s00203-024-04066-5
Abstract: Wild-type Lactococcus lactis strain LAC460 secretes prophage-encoded bacteriocin-like lysin LysL, which kills some Lactococcus strains, but has no lytic effect on the producer. LysL carries two N-terminal enzymatic active domains (EAD), and an unknown C-terminus… read more here.
Keywords: lac460; hgfpuv cbdlysl; lysin; lactis ... See more keywords
Promoting acid resistance and nisin yield of Lactococcus lactis F44 by genetically increasing D-Asp amidation level inside cell wall
Sign Up to like & getrecommendations! Published in 2017 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-017-8365-7
Abstract: Nisin fermentation by Lactococcus lactis requires a low pH to maintain a relatively higher nisin activity. However, the acidic environment will result in cell arrest, and eventually decrease the relative nisin production. Hence, constructing an… read more here.
Keywords: lactis; asp amidation; f44; cell wall ... See more keywords
Production of controlled molecular weight hyaluronic acid by glucostat strategy using recombinant Lactococcus lactis cultures
Sign Up to like & getrecommendations! Published in 2019 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-019-09769-0
Abstract: Hyaluronic acid (HA) is a biopolymer with wide biomedical and cosmetic applications, wherein the molecular weight of HA (MWHA) is an important quality parameter that determines its suitability for the targeted application. To produce HA… read more here.
Keywords: lactis; mwha; production; weight ... See more keywords
Enhancement of acetyl-CoA by acetate co-utilization in recombinant Lactococcus lactis cultures enables the production of high molecular weight hyaluronic acid
Sign Up to like & getrecommendations! Published in 2019 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-019-09987-6
Abstract: The molecular weight of hyaluronic acid (HA) is a critical property which determines its usage in various biomedical applications. This study investigates the correlation between the availability of a critical cofactor, acetyl-CoA, the concentration of… read more here.
Keywords: lactis; molecular weight; acetyl coa; coa ... See more keywords
Profiling electric signals of electrogenic probiotic bacteria using self-attention analysis
Sign Up to like & getrecommendations! Published in 2025 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-025-13425-1
Abstract: We fabricated a self-assembled electric circuit to detect the electrical signals produced by two electrogenic probiotic bacteria [Leuconostoc mesenteroides (L. mesenteroides) and Lactococcus lactis (L. lactis)] on chicken egg chorioallantoic membranes as well as in… read more here.
Keywords: probiotic bacteria; electrogenic probiotic; electrical signals; lactis ... See more keywords