Diversity in kinetics correlated with structure in nano body-stabilized LacY
Sign Up to like & getrecommendations! Published in 2020 at "PLoS ONE"
DOI: 10.1371/journal.pone.0232846
Abstract: The structure of lactose permease, stabilized in a periplasmic open conformation by two Gly to Trp replacements (LacYww) and complexed with a nanobody directed against this conformation, provides the highest resolution structure of the symporter.… read more here.
Keywords: nanobody; open conformation; structure; lacy ... See more keywords
Enhanced electrochemical measurement of β-galactosidase activity in whole cells by coexpression of lactose permease, LacY.
Sign Up to like & getrecommendations! Published in 2022 at "BioTechniques"
DOI: 10.2144/btn-2022-0090
Abstract: Whole-cell biosensing links the sensing and computing capabilities of microbes to the generation of a detectable reporter. Whole cells enable dynamic biological computation (filtered noise, amplified signals, logic gating etc.). Enzymatic reporters enable in situ… read more here.
Keywords: lacy; coexpression lactose; lactose permease; whole cells ... See more keywords