GLUT Characterization Using Frog Xenopus laevis Oocytes.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7507-5_4
Abstract: Xenopus laevis oocytes are a useful heterologous expression system for expressing glucose transporters (GLUTs) and examining their functions. In this chapter, we provide a detailed protocol on oocyte extraction and preparation for GLUT9 protein expression.… read more here.
Keywords: characterization using; xenopus laevis; glut characterization; laevis oocytes ... See more keywords
In Cell NMR Spectroscopy: Investigation of G-Quadruplex Structures Inside Living Xenopus laevis Oocytes.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9666-7_25
Abstract: G-quadruplexes are inherently polymorphic nucleic acid structures. Their folding topology depends on the nucleic acid primary sequence and on physical-chemical environmental factors. Hence, it remains unclear if a G-quadruplex topology determined in the test tube… read more here.
Keywords: xenopus laevis; laevis oocytes; topology; quadruplex structures ... See more keywords
Different efficiency of auxiliary/chaperone proteins to promote the functional reconstitution of honeybee glutamate and acetylcholine receptors in Xenopus laevis oocytes
Sign Up to like & getrecommendations! Published in 2022 at "Insect Molecular Biology"
DOI: 10.1111/imb.12791
Abstract: Heterologous expression systems (e.g., Xenopus laevis oocytes) are useful to study the biophysical properties and pharmacology of ionotropic receptors such as ionotropic glutamate (iGLuRs) and nicotinic acetylcholine (nAChRs) receptors. However, insect receptors often require the… read more here.
Keywords: xenopus laevis; chaperone; expression; proteins promote ... See more keywords
Characterization of Transport Activity of SLC11 Transporters in Xenopus laevis Oocytes by Fluorophore Quenching
Sign Up to like & getrecommendations! Published in 2021 at "SLAS Discovery"
DOI: 10.1177/24725552211004123
Abstract: Membrane proteins are involved in different physiological functions and are the target of pharmaceutical and abuse drugs. Xenopus laevis oocytes provide a powerful heterologous expression system for functional studies of these proteins. Typical experiments investigate… read more here.
Keywords: xenopus laevis; characterization transport; transport; laevis oocytes ... See more keywords