Pif1 is essential for efficient replisome progression through lagging strand G-quadruplex DNA secondary structures
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DOI: 10.1093/nar/gky1065
Abstract: Abstract Pif1 DNA helicase is a potent unwinder of G-quadruplex (G4) structures in vitro and functions to maintain genome stability at G4 sequences in Saccharomyces cerevisiae. Here, we developed and utilized a live-cell imaging approach… read more here.
Keywords: pif1 essential; lagging strand; replisome progression; progression ... See more keywords
Processing of eukaryotic Okazaki fragments by redundant nucleases can be uncoupled from ongoing DNA replication in vivo
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DOI: 10.1093/nar/gky1242
Abstract: Abstract Prior to ligation, each Okazaki fragment synthesized on the lagging strand in eukaryotes must be nucleolytically processed. Nuclease cleavage takes place in the context of 5′ flap structures generated via strand-displacement synthesis by DNA… read more here.
Keywords: lagging strand; synthesis; okazaki fragments; dna ... See more keywords
Replicative DNA polymerases promote active displacement of SSB proteins during lagging strand synthesis
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DOI: 10.1093/nar/gkz249
Abstract: Abstract Genome replication induces the generation of large stretches of single-stranded DNA (ssDNA) intermediates that are rapidly protected by single-stranded DNA-binding (SSB) proteins. To date, the mechanism by which tightly bound SSBs are removed from… read more here.
Keywords: dna; polymerase; lagging strand; ssb proteins ... See more keywords
Bacillus subtilis RadA/Sms-Mediated Nascent Lagging-Strand Unwinding at Stalled or Reversed Forks Is a Two-Step Process: RadA/Sms Assists RecA Nucleation, and RecA Loads RadA/Sms
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DOI: 10.1101/2023.01.24.525305
Abstract: Replication fork rescue requires Bacillus subtilis RecA, its negative (SsbA) and positive (RecO) mediators, and fork-processing (RadA/Sms). To understand how they work to promote fork remodeling, reconstituted branched replication intermediates were used. We show that… read more here.
Keywords: nascent lagging; lagging strand; reca; rada sms ... See more keywords
Lagging Strand Initiation Processes in DNA Replication of Eukaryotes—Strings of Highly Coordinated Reactions Governed by Multiprotein Complexes
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DOI: 10.3390/genes14051012
Abstract: In their influential reviews, Hanahan and Weinberg coined the term ‘Hallmarks of Cancer’ and described genome instability as a property of cells enabling cancer development. Accurate DNA replication of genomes is central to diminishing genome… read more here.
Keywords: replication; lagging strand; synthesis; initiation ... See more keywords