Efficient precise integration of large DNA sequences with 3'-overhang dsDNA donors using CRISPR/Cas9.
Sign Up to like & getrecommendations! Published in 2023 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2221127120
Abstract: CRISPR/Cas9 genome-editing tools have tremendously boosted our capability of manipulating the eukaryotic genomes in biomedical research and innovative biotechnologies. However, the current approaches that allow precise integration of gene-sized large DNA fragments generally suffer from… read more here.
Keywords: crispr cas9; dna; integration; large dna ... See more keywords
Approaches for introducing large DNA molecules into bacterial cells.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of applied microbiology"
DOI: 10.1093/jambio/lxad059
Abstract: Engineering of the bacterial genome plays a key role in systems biology and synthetic biology. Genetic engineering of the bacterial genome involves the design and synthesis of large DNA molecules. However, functional studies of the… read more here.
Keywords: molecules bacterial; large dna; dna molecules; biology ... See more keywords
Design, optimization and analysis of large DNA and RNA nanostructures through interactive visualization, editing and molecular simulation
Sign Up to like & getrecommendations! Published in 2020 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkaa417
Abstract: Abstract This work seeks to remedy two deficiencies in the current nucleic acid nanotechnology software environment: the lack of both a fast and user-friendly visualization tool and a standard for structural analyses of simulated systems.… read more here.
Keywords: rna nanostructures; dna; analysis; large dna ... See more keywords
A New Efficient Algorithm for Quorum Planted Motif Search on Large DNA Datasets
Sign Up to like & getrecommendations! Published in 2019 at "IEEE Access"
DOI: 10.1109/access.2019.2940115
Abstract: Quorum planted ( $l, d$ ) motif search (qPMS) is a challenging computational problem in bioinformatics, mainly for the identification of regulatory elements such as transcription factor binding sites in DNA sequences. Large DNA datasets… read more here.
Keywords: tex math; inline formula; large dna; dna datasets ... See more keywords
Acetosyringone treatment duration affects large T-DNA molecule transfer to rice callus
Sign Up to like & getrecommendations! Published in 2018 at "BMC Biotechnology"
DOI: 10.1186/s12896-018-0459-5
Abstract: BackgroundLarge T-DNA fragment transfer has long been a problem for Agrobacterium-mediated transformation. Although vector systems, such as the BIBAC series, were successfully developed for the purpose, low transformation efficiencies were consistently observed.ResultsTo gain insights of… read more here.
Keywords: dna; large dna; transformation; transfer rice ... See more keywords
Programmable RNA-Guided Large DNA Transgenesis by CRISPR/Cas9 and Site-Specific Integrase Bxb1
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2022.910151
Abstract: The inability to insert large DNA constructs into the genome efficiently and precisely is a key challenge in genomic engineering. Random transgenesis, which is widely used, lacks precision, and comes with a slew of drawbacks.… read more here.
Keywords: cas9 site; crispr cas9; dna; cas9 ... See more keywords