CRISPR/Cpf1 facilitated large fragment deletion in Saccharomyces cerevisiae
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Basic Microbiology"
DOI: 10.1002/jobm.201800195
Abstract: In this study, we focused on the applicability of CRISPR/Cpf1 in genome simplification of Saccharomyces cerevisiae and established a CRISPR/Cpf1 assisted method for rapid markerless large fragment deletion to facilitate laboratory evolution of geome of… read more here.
Keywords: fragment deletion; crispr cpf1; saccharomyces cerevisiae; large fragment ... See more keywords
Embryo-Based Large Fragment Knock-in in Mammals: Why, How and What’s Next
Sign Up to like & getrecommendations! Published in 2020 at "Genes"
DOI: 10.3390/genes11020140
Abstract: Endonuclease-mediated genome editing technologies, most notably CRISPR/Cas9, have revolutionized animal genetics by allowing for precise genome editing directly through embryo manipulations. As endonuclease-mediated model generation became commonplace, large fragment knock-in remained one of the most… read more here.
Keywords: knock; fragment knock; large fragment; embryo based ... See more keywords
Large Fragment InDels Reshape Genome Structure of Porcine Alveolar Macrophage 3D4/21 Cells
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DOI: 10.3390/genes13091515
Abstract: The porcine monomyeloid cell line, or 3D4/21 cells, is an effective tool to study the immune characteristics and virus infection mechanism of pigs. Due to the introduction of the neomycin resistance gene and the SV40… read more here.
Keywords: fragment indels; large fragment; structure; 3d4 cells ... See more keywords
Systematic Large Fragment Deletions in the Genome of Synechococcus elongatus and the Consequent Changes in Transcriptomic Profiles
Sign Up to like & getrecommendations! Published in 2023 at "Genes"
DOI: 10.3390/genes14051091
Abstract: Genome streamlining, as a natural process in the evolution of microbes, has become a common approach for generating ideal chassis cells for synthetic biology studies and industrial applications. However, systematic genome reduction remains a bottleneck… read more here.
Keywords: systematic large; large fragment; genome; synechococcus elongatus ... See more keywords