Combining the "Sibling Technologies" of Laser Capture Microdissection and Reverse Phase Protein Microarrays.
Sign Up to like & getrecommendations! Published in 2019 at "Advances in experimental medicine and biology"
DOI: 10.1007/978-981-32-9755-5_6
Abstract: Reverse phase protein microarrays (RPPA) and laser capture microdissection (LCM) are "sibling" technologies that originated from the same laboratory to overcome the challenge of quantifying low-abundance proteins in heterogeneous tissues. Combining both technologies provides both… read more here.
Keywords: laser capture; sibling technologies; protein microarrays; reverse phase ... See more keywords
Laser capture microdissection: techniques and applications in liver diseases
Sign Up to like & getrecommendations! Published in 2019 at "Hepatology International"
DOI: 10.1007/s12072-018-9917-3
Abstract: Routine transcriptomic and proteomic analysis are usually performed at a whole organ or tissue level. These approaches provide an average readout of all cell types present within the tissue but do not allow differentiating the… read more here.
Keywords: capture microdissection; laser capture; liver diseases; tissue ... See more keywords
Laser capture microdissection on formalin-fixed and paraffin-embedded renal transplanted biopsies: Technical perspectives for clinical practice application.
Sign Up to like & getrecommendations! Published in 2020 at "Experimental and molecular pathology"
DOI: 10.1016/j.yexmp.2020.104516
Abstract: Renal biopsy (RBx) is an essential tool in the diagnostic and therapeutic process of most native kidney diseases and in the renal transplanted graft. Laser capture microdissection (LCM), combined with molecular biology, might improve the… read more here.
Keywords: laser capture; fixed paraffin; paraffin embedded; renal transplanted ... See more keywords
Shotgun Lipidomics Combined with Laser Capture Microdissection: A Tool To Analyze Histological Zones in Cryosections of Tissues.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.8b02004
Abstract: Shotgun analysis provides a quantitative snapshot of the lipidome composition of cells, tissues, or model organisms; however, it does not elucidate the spatial distribution of lipids. Here we demonstrate that shotgun analysis could quantify low-picomole… read more here.
Keywords: capture microdissection; laser capture; histological zones; cryosections tissues ... See more keywords
Mouse Paneth Cell-Enriched Proteome Enabled by Laser Capture Microdissection.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.2c00311
Abstract: Paneth cells are antimicrobial peptide-secreting cells located at the base of the crypts of the small intestine. The proteome of Paneth cells is not well defined because of their coexistence with stem cells, making it… read more here.
Keywords: capture microdissection; cell; laser capture; paneth cells ... See more keywords
Laser Capture Microdissection in the Spatial Analysis of Epigenetic Modifications in Skin: A Comprehensive Review
Sign Up to like & getrecommendations! Published in 2022 at "Oxidative Medicine and Cellular Longevity"
DOI: 10.1155/2022/4127238
Abstract: Each cell in the body contains an intricate regulation for the expression of its relevant DNA. While every cell in a multicellular organism contains identical DNA, each tissue-specific cell expresses a different set of active… read more here.
Keywords: cell; review; laser capture; pathology ... See more keywords
Laser Capture Microdissection - A Breakthrough Technology in Head and Neck Diagnostics
Sign Up to like & getrecommendations! Published in 2023 at "Bangladesh Journal of Medical Science"
DOI: 10.3329/bjms.v22i20.66308
Abstract: Laser capture microdissection (LCM) is a technique in which, from a heterogenous tissue specimen some pure cell populations are isolated. This procedure can capture and target the desired number of cells that are required. A… read more here.
Keywords: capture microdissection; laser capture; microdissection breakthrough; capture ... See more keywords
Robust Acquisition of Spatial Transcriptional Programs in Tissues With Immunofluorescence-Guided Laser Capture Microdissection
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Cell and Developmental Biology"
DOI: 10.3389/fcell.2022.853188
Abstract: The functioning of tissues is fundamentally dependent upon not only the phenotypes of the constituent cells but also their spatial organization in the tissue, as local interactions precipitate intra-cellular events that often lead to changes… read more here.
Keywords: capture microdissection; laser capture; transcriptional programs; immunofluorescence guided ... See more keywords
Global-feature of autoimmune glomerulonephritis using proteomic analysis of laser capture microdissected glomeruli
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Immunology"
DOI: 10.3389/fimmu.2023.1131164
Abstract: Background IgA nephropathy (IgAN), (LN), membranous nephropathy (MN), and minimal change nephropathy (MCN) are all belonged to autoimmune glomerulonephritis. This study aimed to identify the specific proteomic characteristics of the four GNs diseases in order… read more here.
Keywords: global feature; gns diseases; laser capture; autoimmune glomerulonephritis ... See more keywords
Optimized Method for Robust Transcriptome Profiling of Minute Tissues Using Laser Capture Microdissection and Low-Input RNA-Seq
Sign Up to like & getrecommendations! Published in 2017 at "Frontiers in Molecular Neuroscience"
DOI: 10.3389/fnmol.2017.00185
Abstract: Obtaining high quality RNA from complex biological tissues, such as the brain, is needed for establishing high-fidelity cell-type specific transcriptomes. Although combining genetic labeling techniques with laser capture microdissection (LCM) is generally sufficient, concerns over… read more here.
Keywords: laser capture; rna; method; capture microdissection ... See more keywords
A high-efficiency trichome collection system by laser capture microdissection
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2022.985969
Abstract: Trichomes, which are classified as glandular or non-glandular, are hair-like epidermal structures that are present on aerial parts of most plant species. Glandular secretory trichomes (GSTs) have the capacity to secrete and store specialized metabolites,… read more here.
Keywords: capture microdissection; laser capture; efficiency trichome; high efficiency ... See more keywords