Comparative analysis in different organs and tissue-specific metabolite profiling of Atractylodes lancea from four regions by GC-MS and Laser Microdissection.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of separation science"
DOI: 10.1002/jssc.202100924
Abstract: Traditional Chinese medicine made from the rhizome of Atractylodes lancea (Thunb.) DC. (Compositae), known as Cangzhu. In this study, gas chromatography-mass spectrometry was used to identify and quantify the volatile oils of different organs of… read more here.
Keywords: lancea four; atractylodes lancea; different organs; laser microdissection ... See more keywords
Laser Microdissection as a Useful Tool to Study Gene Expression in Plant and Fungal Partners in AM Symbiosis.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0603-2_13
Abstract: Laser microdissection (LMD) technology has been widely applied to plant tissues, offering novel information on the role of different cell-type populations during plant-microbe interactions. In this chapter, protocols to apply the LMD approach to study… read more here.
Keywords: plant fungal; plant; gene expression; study ... See more keywords
LC-MS/MS imaging with thermal film-based laser microdissection
Sign Up to like & getrecommendations! Published in 2017 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-017-0739-2
Abstract: AbstractMass spectrometry (MS) imaging is a useful tool for direct and simultaneous visualization of specific molecules. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is used to evaluate the abundance of molecules in tissues using sample homogenates. To… read more here.
Keywords: film based; thermal film; based laser; film ... See more keywords
Transcriptional profiling of cork oak phellogenic cells isolated by laser microdissection
Sign Up to like & getrecommendations! Published in 2017 at "Planta"
DOI: 10.1007/s00425-017-2786-5
Abstract: AbstractMain conclusionThe phenylpropanoid pathway impacts the cork quality development. In cork of bad quality, the flavonoid route is favored, whereas in good quality, cork lignin and suberin production prevails. Cork oaks develop a thick cork… read more here.
Keywords: quality; quality cork; pathway; cork ... See more keywords
Spatiotemporal plant hormone analysis from cryosections using laser microdissection-liquid chromatography-mass spectrometry.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of plant research"
DOI: 10.1007/s10265-021-01360-x
Abstract: Laser microdissection (LMD) is used for isolating specific regions or single cells from a wide variety of tissue samples under direct microscopic observation. The LMD method enables the harvest of the cells of interest in… read more here.
Keywords: plant; analysis; plant hormone; liquid chromatography ... See more keywords
Laser microdissection: A powerful tool for genomics at cell level.
Sign Up to like & getrecommendations! Published in 2018 at "Molecular aspects of medicine"
DOI: 10.1016/j.mam.2017.09.003
Abstract: Laser microdissection (LM) has become widely democratized over the last fifteen years. Instruments have evolved to offer more powerful and efficient lasers as well as new options for sample collection and preparation. Technological evolutions have… read more here.
Keywords: powerful tool; cell level; microdissection; laser microdissection ... See more keywords
A rapid preparation procedure for laser microdissection-mediated harvest of plant tissues for gene expression analysis
Sign Up to like & getrecommendations! Published in 2019 at "Plant Methods"
DOI: 10.1186/s13007-019-0471-3
Abstract: BackgroundGene expression changes that govern essential biological processes can occur at the cell-specific level. To gain insight into such events, laser microdissection is applied to cut out specific cells or tissues from which RNA for… read more here.
Keywords: gene expression; laser microdissection; plant;
A protocol for laser microdissection (LMD) followed by transcriptome analysis of plant reproductive tissue in phylogenetically distant angiosperms
Sign Up to like & getrecommendations! Published in 2019 at "Plant Methods"
DOI: 10.1186/s13007-019-0536-3
Abstract: BackgroundPlant development is controlled by the action of many, often connected gene regulatory networks. Differential gene expression controlled by internal and external cues is a major driver of growth and time specific differentiation in plants.… read more here.
Keywords: quality; plant; analysis; tissue ... See more keywords
The Cause of Death of a Child in the 18th Century Solved by Bone Microbiome Typing Using Laser Microdissection and Next Generation Sequencing
Sign Up to like & getrecommendations! Published in 2017 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms18010109
Abstract: The history of medicine abounds in cases of mysterious deaths, especially by infectious diseases, which were probably unresolved because of the lack of knowledge and of appropriate technology. The aim of this study was to… read more here.
Keywords: microdissection next; laser; 18th century; cause death ... See more keywords