Discovery and Characterization of Rubrinodin Provide Clues into the Evolution of Lasso Peptides.
Sign Up to like & getrecommendations! Published in 2022 at "Biochemistry"
DOI: 10.1021/acs.biochem.2c00029
Abstract: Lasso peptides are unique natural products that comprise a class of ribosomally synthesized and post-translationally modified peptides. Their defining three-dimensional structure is a lariat knot, in which the C-terminal tail is threaded through a macrolactam… read more here.
Keywords: lasso peptides; lasso; lasso peptide; rubrinodin ... See more keywords
Kinetics of Aspartimide Formation and Hydrolysis in Lasso Peptide Lihuanodin.
Sign Up to like & getrecommendations! Published in 2023 at "Biochemistry"
DOI: 10.1021/acs.biochem.2c00707
Abstract: Aspartimides are notorious as undesired side products in solid-phase peptide synthesis and in pharmaceutical formulations. However, we have discovered several ribosomally synthesized and post-translationally modified peptides (RiPPs) in which aspartimide is installed intentionally via enzymatic… read more here.
Keywords: lasso peptide; kinetics aspartimide; formation; peptide lihuanodin ... See more keywords
Discovery and Bioactivity of the Novel Lasso Peptide Microcin Y.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.1c02659
Abstract: Lasso peptides, a class of ribosomally synthesized and post-translationally modified peptides (RiPPs) secreted by bacteria, have antimicrobial activity. Here, a novel lasso peptide, microcin Y (MccY), was discovered and characterized. The gene cluster for MccY… read more here.
Keywords: discovery bioactivity; peptide; peptide microcin; lasso peptide ... See more keywords
LassoHTP: A High-Throughput Computational Tool for Lasso Peptide Structure Construction and Modeling
Sign Up to like & getrecommendations! Published in 2023 at "Journal of chemical information and modeling"
DOI: 10.1021/acs.jcim.2c00945
Abstract: Lasso peptides are a subclass of ribosomally synthesized and post-translationally modified peptides with a slipknot conformation. With superior thermal stability, protease resistance, and antimicrobial activity, lasso peptides are promising candidates for bioengineering and pharmaceutical applications.… read more here.
Keywords: lasso peptide; peptide; high throughput; peptide structure ... See more keywords
Investigation of the Biosynthesis of the Lasso Peptide Chaxapeptin Using an E. coli-Based Production System.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of natural products"
DOI: 10.1021/acs.jnatprod.8b00392
Abstract: Lasso peptides are natural products belonging to the family of ribosomally synthesized and posttranslationally modified peptides (RiPPs) and are defined by their unique topology. Even though lasso peptide biosynthetic gene clusters are found in many… read more here.
Keywords: chaxapeptin; lasso peptides; lasso peptide; production system ... See more keywords
High-Throughput Screen Reveals the Structure–Activity Relationship of the Antimicrobial Lasso Peptide Ubonodin
Sign Up to like & getrecommendations! Published in 2022 at "ACS Central Science"
DOI: 10.1021/acscentsci.2c01487
Abstract: The Burkholderia cepacia complex (Bcc) is a group of bacteria including several opportunistic human pathogens. Immunocompromised individuals and cystic fibrosis patients are especially vulnerable to serious infections by these bacteria, motivating the search for compounds… read more here.
Keywords: structure activity; throughput screen; lasso peptide; high throughput ... See more keywords
CanE, an Iron/2-Oxoglutarate-dependent Lasso Peptide Hydroxylase from Streptomyces canus.
Sign Up to like & getrecommendations! Published in 2020 at "ACS chemical biology"
DOI: 10.1021/acschembio.0c00109
Abstract: Lasso peptides are a class of ribosomally-synthesized and post-translationally modified peptides (RiPPs) that feature a unique lariat-knot topology. Canucin A, a post-translationally hydroxylated lasso peptide, was recently discovered via activation of its otherwise silent biosynthetic… read more here.
Keywords: streptomyces canus; iron oxoglutarate; oxoglutarate dependent; lasso peptide ... See more keywords
Comprehensive Mutational Analysis of the Lasso Peptide Klebsidin.
Sign Up to like & getrecommendations! Published in 2022 at "ACS chemical biology"
DOI: 10.1021/acschembio.2c00148
Abstract: Antibiotic resistance is a growing threat to public health, making the development of antibiotics of critical importance. One promising class of potential new antibiotics are ribosomally synthesized and post-translationally modified peptides (RiPPs), which include klebsidin,… read more here.
Keywords: lasso peptide; analysis lasso; klebsidin; mutational analysis ... See more keywords
Phenotype-Guided Comparative Genomics Identifies the Complete Transport Pathway of the Antimicrobial Lasso Peptide Ubonodin in Burkholderia.
Sign Up to like & getrecommendations! Published in 2022 at "ACS chemical biology"
DOI: 10.1021/acschembio.2c00420
Abstract: New antibiotics are needed as bacterial infections continue to be a leading cause of death, but efforts to develop compounds with promising antibacterial activity are hindered by a poor understanding of─and limited strategies for elucidating─their… read more here.
Keywords: peptide ubonodin; lasso peptide; peptide; phenotype guided ... See more keywords
Cell-Free Biosynthesis to Evaluate Lasso Peptide Formation and Enzyme-Substrate Tolerance.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.1c01452
Abstract: Lasso peptides are ribosomally synthesized and post-translationally modified peptide (RiPP) natural products that display a unique lariat-like, threaded conformation. Owing to a locked three-dimensional structure, lasso peptides can be unusually stable toward heat and proteolytic… read more here.
Keywords: lasso peptides; cfb; lasso peptide; cell free ... See more keywords
Heterologous Expression of a Cryptic Gene Cluster from Streptomyces leeuwenhoekii C34T Yields a Novel Lasso Peptide, Leepeptin
Sign Up to like & getrecommendations! Published in 2019 at "Applied and Environmental Microbiology"
DOI: 10.1128/aem.01752-19
Abstract: Recent developments in genome sequencing combined with bioinformatic analysis have revealed that actinomycetes contain a plethora of unexpected BGCs and thus have the potential to produce many more natural products than previously thought. This reflects… read more here.
Keywords: expression; lasso peptide; heterologous expression; production ... See more keywords