Dynamic Ca2+ imaging with a simplified lattice light-sheet microscope: A sideways view of subcellular Ca2+ puffs.
Sign Up to like & getrecommendations! Published in 2018 at "Cell calcium"
DOI: 10.1016/j.ceca.2017.11.005
Abstract: We describe the construction of a simplified, inexpensive lattice light-sheet microscope, and illustrate its use for imaging subcellular Ca2+ puffs evoked by photoreleased i-IP3 in cultured SH-SY5Y neuroblastoma cells loaded with the Ca2+ probe Cal520.… read more here.
Keywords: ca2 puffs; microscope; lattice light; ca2 ... See more keywords
Characterization, comparison, and optimization of lattice light sheets
Sign Up to like & getrecommendations! Published in 2022 at "Science Advances"
DOI: 10.1101/2022.07.30.502108
Abstract: Lattice light sheet microscopy excels at the non-invasive imaging of three-dimensional (3D) dynamic processes at high spatiotemporal resolution within cells and developing embryos. Recently, several papers have called into question the performance of lattice light… read more here.
Keywords: light sheet; light sheets; microscopy; lattice light ... See more keywords
Active image optimization for lattice light sheet microscopy in thick samples.
Sign Up to like & getrecommendations! Published in 2022 at "Biomedical optics express"
DOI: 10.1364/boe.471757
Abstract: Lattice light-sheet microscopy (LLSM) is a very efficient technique for high resolution 3D imaging of dynamic phenomena in living biological samples. However, LLSM imaging remains limited in depth due to optical aberrations caused by sample-based… read more here.
Keywords: light sheet; microscopy; lattice light; optimization ... See more keywords
Enhanced axial resolution of lattice light sheet microscopy by fluorescence differential detection.
Sign Up to like & getrecommendations! Published in 2022 at "Optics express"
DOI: 10.1364/oe.462516
Abstract: Lattice light-sheet microscopy (LLSM) is promising in long-term biological volumetric imaging due to its high spatiotemporal resolution and low phototoxicity. However, three-dimensional (3D) isotropic spatial resolution remains an unmet goal in LLSM because of its… read more here.
Keywords: microscopy; lattice light; axial resolution; llsm ... See more keywords