Detection and quantification of transition metal leaching in metal affinity chromatography with hydroxynaphthol blue.
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DOI: 10.1016/j.ab.2019.113347
Abstract: The widespread use of immobilized metal-affinity chromatography (IMAC) for fast and efficient purification of recombinant proteins has brought potentially toxic transition elements into common laboratory usage. However, there are few studies on the leaching of… read more here.
Keywords: biochemistry; metal affinity; metal; affinity chromatography ... See more keywords