Construction and characterization of the full-length cDNA of an infectious clone of emerging porcine teschovirus-2.
Sign Up to like & getrecommendations! Published in 2022 at "Pathogens and disease"
DOI: 10.1093/femspd/ftac033
Abstract: Porcine teschovirus (PTV) is a causative agent of polioencephalomyelitis, encephalomyelitis, reproductive disorders and gastrointestinal and respiratory diseases in swine. In the present study, the PTV2 GX/2020 strain was isolated from pig intestinal tissue through the… read more here.
Keywords: cdna infectious; full length; length cdna; porcine teschovirus ... See more keywords
Improved Nanopore full-length cDNA sequencing by PCR-suppression
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Genetics"
DOI: 10.3389/fgene.2022.1031355
Abstract: Full-length transcript sequencing remains a main goal of RNA sequencing. However, even the application of long-read sequencing technologies such as Oxford Nanopore Technologies still fail to yield full-length transcript sequencing for a significant portion of… read more here.
Keywords: full length; length cdna; cdna sequencing; pcr suppression ... See more keywords
A Reverse Genetics System for Zika Virus Based on a Simple Molecular Cloning Strategy
Sign Up to like & getrecommendations! Published in 2018 at "Viruses"
DOI: 10.3390/v10070368
Abstract: The Zika virus (ZIKV) has recently attracted major research interest as infection was unexpectedly associated with neurological manifestations in developing foetuses and with Guillain-Barré syndrome in infected adults. Understanding the underlying molecular mechanisms requires reverse… read more here.
Keywords: length cdna; full length; cdna clones; genetics ... See more keywords