Evaluation of lentiviral vector stability and development of ion exchange purification processes
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Progress"
DOI: 10.1002/btpr.3286
Abstract: In this manuscript, we employ parallel batch stability and chromatographic screens in concert with linear and step gradient experiments to develop a high yield, HCP clearance anion exchange capture process for lentiviral vector (LVV) purification.… read more here.
Keywords: purification; lvv; exchange; lentiviral vector ... See more keywords
In vivo screens using a selective CRISPR antigen removal lentiviral vector system reveal immune dependencies in renal cell carcinoma.
Sign Up to like & getrecommendations! Published in 2021 at "Immunity"
DOI: 10.1016/j.immuni.2021.01.001
Abstract: CRISPR-Cas9 genome engineering has increased the pace of discovery for immunology and cancer biology, revealing potential therapeutic targets and providing insight into mechanisms underlying resistance to immunotherapy. However, endogenous immune recognition of Cas9 has limited… read more here.
Keywords: system; crispr; antigen; crispr antigen ... See more keywords
Generation of a lentiviral vector system to efficiently express bioactive recombinant human prolactin hormones
Sign Up to like & getrecommendations! Published in 2020 at "Molecular and Cellular Endocrinology"
DOI: 10.1016/j.mce.2019.110605
Abstract: The contribution of the pleiotropic hormone Prolactin (PRL) to several physiological and pathological processes is still unknown. To clarify the role of PRL in these processes during the last decade, different human PRL antagonists have… read more here.
Keywords: vector system; prolactin; system efficiently; hprl ... See more keywords
A lentiviral vector bearing a reverse intron demonstrates superior expression of both proteins and microRNAs
Sign Up to like & getrecommendations! Published in 2017 at "RNA Biology"
DOI: 10.1080/15476286.2017.1334755
Abstract: ABSTRACT While lentiviral expression vectors are widely used in many facets of molecular biology, due to their ability to stably express heterologous genes in both dividing and non-dividing cells, they suffer from the disadvantage that… read more here.
Keywords: vector; intron; expression; biology ... See more keywords
Improving the Transduction of Bone Marrow–Derived Cells with an Integrase-Defective Lentiviral Vector
Sign Up to like & getrecommendations! Published in 2018 at "Human Gene Therapy Methods"
DOI: 10.1089/hgtb.2017.082
Abstract: In lentiviral vector (LV) applications where transient transgene expression is sufficient, integrase-defective lentiviral vectors (IDLVs) are beneficial for reducing the potential for off-target effects associated with insertional mutagenesis. It was previously demonstrated that human RPE65… read more here.
Keywords: idlv3 rpe65; expression; rpe65; transduction ... See more keywords
Efficient Gene Transfer to Kidney Using a Lentiviral Vector Pseudotyped with Zika Virus Envelope Glycoprotein.
Sign Up to like & getrecommendations! Published in 2022 at "Human gene therapy"
DOI: 10.1089/hum.2022.053
Abstract: Gene therapy's entrance into clinical settings has made it an ever more attractive field of study for various diseases. However, relatively little progress has been made in targeting kidney diseases due to poor gene delivery… read more here.
Keywords: kidney; lentiviral vector; gene therapy; vector ... See more keywords
Directly injected lentiviral vector–based T cell vaccine protects mice against acute and chronic viral infection
Sign Up to like & getrecommendations! Published in 2022 at "JCI Insight"
DOI: 10.1172/jci.insight.161598
Abstract: Lentiviral vector–based dendritic cell vaccines induce protective T cell responses against viral infection and cancer in animal models. In this study, we tested whether preventative and therapeutic vaccination could be achieved by direct injection of… read more here.
Keywords: viral infection; cell; vector based; lentiviral vector ... See more keywords
Construction of recombinant lentiviral vector containing human stem cell leukemia gene and its expression in interstitial cells of cajal
Sign Up to like & getrecommendations! Published in 2019 at "Open Life Sciences"
DOI: 10.1515/biol-2020-0010
Abstract: Abstract This study aims to construct recombinant lentiviral vectors containing the human stem cell leukemia (SCL) gene and investigate their in vitro transfection efficiency in Interstitial Cells of Cajal (ICC) of guinea pig bladders. In… read more here.
Keywords: containing human; recombinant lentiviral; gene; human stem ... See more keywords
The Lentiviral Vector Pseudotyped by Modified Rabies Glycoprotein Does Not Cause Reactive Gliosis and Neurodegeneration in Rat Hippocampus
Sign Up to like & getrecommendations! Published in 2019 at "Iranian biomedical journal"
DOI: 10.29252/ibj.23.5.3
Abstract: Background A human immunodeficiency virus type 1 (HIV-1)-based lentiviral vector (LV) pseudotyped by a variant of rabies envelope glycoprotein, FUG-B2, has previously been prepared and used in transfection of hippocampal CA1 ("Cornu Ammonis" area 1)… read more here.
Keywords: vector; reactive gliosis; vector pseudotyped; rat hippocampus ... See more keywords
Effective Amelioration of Liver Fibrosis Through Lentiviral Vector Carrying Toxoplasma gondii gra15II in Murine Model
Sign Up to like & getrecommendations! Published in 2018 at "Frontiers in Immunology"
DOI: 10.3389/fimmu.2018.01572
Abstract: Our previous investigations indicated that in vitro polarization of mouse macrophages by Toxoplasma gondii type II strain dense granule protein 15 (GRA15II), one of the genotype-associated effectors of T. gondii, induced the phenotypes of classically… read more here.
Keywords: gra15ii; vector carrying; toxoplasma gondii; amelioration liver ... See more keywords
BCSG1 siRNA delivered by lentiviral vector suppressed proliferation and migration of MDA-MB-231 cells.
Sign Up to like & getrecommendations! Published in 2018 at "International journal of molecular medicine"
DOI: 10.3892/ijmm.2017.3355
Abstract: Breast cancer-specific gene 1 (BCSG1), also referred to as γ-synuclein (SNCG), is highly expressed in human infiltrating breast carcinomas, but not in normal or benign breast tissue. The present study aimed to evaluate the effects of… read more here.
Keywords: bcsg1 sirna; proliferation; delivered lentiviral; sirna delivered ... See more keywords