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   Articles with "levansucrase" as a keyword



Catalytic biosynthesis of levan and short-chain fructooligosaccharides from sucrose-containing feedstocks by employing the levansucrase from Leuconostoc mesenteroides MTCC10508.

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recommendations! Published in 2019 at "International journal of biological macromolecules"

DOI: 10.1016/j.ijbiomac.2019.01.070

Abstract: Levansucrase gene (LmLEVS) was cloned from Leuconostoc mesenteroides MTCC 10508. The heterologous expression and purification of the truncated (TrLmLEVS) gene, lacking the N-terminal signal peptide, was performed in Escherichia coli. The recombinant enzyme (TrLmLEVS) was… read more here.

Keywords: containing feedstocks; levan; biosynthesis; levansucrase ... See more keywords

Successful Manipulation of the Product Spectrum of the Erwinia amylovora Levansucrase by Modifying the Residues around loop1, Loop 3, and Loop 4.

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recommendations! Published in 2022 at "Journal of agricultural and food chemistry"

DOI: 10.1021/acs.jafc.2c07891

Abstract: Levansucrase (LS, EC 2.4.1.10) catalyzes the synthesis of levan by successively transferring the fructosyl moiety from sucrose to an elongated fructan chain. Although the product distribution of LS from Erwinia amylovora (Ea-LS) was studied under… read more here.

Keywords: erwinia amylovora; loop; levansucrase; product spectrum ... See more keywords

Molecular Basis of Pseudomonas syringae pv actinidiae Levansucrase Inhibition by a Multivalent Iminosugar

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recommendations! Published in 2025 at "Journal of Agricultural and Food Chemistry"

DOI: 10.1021/acs.jafc.5c01947

Abstract: Levansucrases are a class of polysaccharide-processing enzymes widely distributed among plant pathogenic bacteria, such as Pseudomonas syringae and Erwinia amylovora. Therefore, the modulation of levansucrase activity could represent a new strategy to reduce the microbial… read more here.

Keywords: levansucrase; inhibition; tpis; syringae actinidiae ... See more keywords

Impaired coordination of nucleophile and increased hydrophobicity in the +1 subsite shift levansucrase activity towards transfructosylation

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recommendations! Published in 2017 at "Glycobiology"

DOI: 10.1093/glycob/cwx050

Abstract: Abstract Bacterial levansucrases produce β(2,6)-linked levan-type polysaccharides using sucrose or sucrose analogs as donor/acceptor substrates. However, the dominant reaction of Bacillus megaterium levansucrase (Bm-LS) is hydrolysis. Single domain levansucrases from Gram-positive bacteria display a wide… read more here.

Keywords: coordination nucleophile; levansucrase; transfructosylation; water ... See more keywords