In‐depth analysis of the Sirtuin 5‐regulated mouse brain malonylome and succinylome using library‐free data‐independent acquisitions
Sign Up to like & getrecommendations! Published in 2022 at "PROTEOMICS"
DOI: 10.1002/pmic.202100371
Abstract: Post‐translational modifications (PTMs) dynamically regulate proteins and biological pathways, typically through the combined effects of multiple PTMs. Lysine residues are targeted for various PTMs, including malonylation and succinylation. However, PTMs offer specific challenges to mass… read more here.
Keywords: data independent; free data; sirtuin; mouse brain ... See more keywords
BoxCar and Library-Free Data-Independent Acquisition Substantially Improve the Depth, Range, and Completeness of Label-Free Quantitative Proteomics.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.1c03338
Abstract: Data-dependent acquisition (DDA) methods are the current standard for quantitative proteomics in many biological systems. However, DDA preferentially measures highly abundant proteins and generates data that is plagued with missing values, requiring extensive imputation. Here,… read more here.
Keywords: data independent; range; quantitative proteomics; independent acquisition ... See more keywords
Definitive Screening Designs to Optimize Library-Free DIA-MS Identification and Quantification of Neuropeptides.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.3c00088
Abstract: Method optimization is crucial for successful mass spectrometry (MS) analysis. However, extensive method assessments, altering various parameters individually, are rarely performed due to practical limitations regarding time and sample quantity. To maximize sample space for… read more here.
Keywords: dia; identification; screening designs; definitive screening ... See more keywords