An accurate bacterial DNA quantification assay for HTS library preparation of human biological samples
Sign Up to like & getrecommendations! Published in 2018 at "ELECTROPHORESIS"
DOI: 10.1002/elps.201800127
Abstract: Sequencing and classification of microbial taxa within forensically relevant biological fluids has the potential for applications in the forensic science and biomedical fields. The quantity of bacterial DNA from human samples is currently estimated based… read more here.
Keywords: bacterial dna; dna; library preparation; quantity ... See more keywords
Small RNA Library Preparation and Illumina Sequencing in Plants.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4899-7708-3_15
Abstract: The discovery of small RNAs in plants and animals almost two decades ago attracted a significant interest towards epigenetic regulation of gene expression and the practical implementation of the gained knowledge in applied studies. New… read more here.
Keywords: small rna; illumina sequencing; rna library; library preparation ... See more keywords
CleanTag Adapters Improve Small RNA Next-Generation Sequencing Library Preparation by Reducing Adapter Dimers.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7514-3_10
Abstract: Next-generation small RNA sequencing is a valuable tool which is increasing our knowledge regarding small noncoding RNAs and their function in regulating genetic information. Library preparation protocols for small RNA have thus far been restricted… read more here.
Keywords: small rna; library preparation; adapter dimer;
Inline index helped in cleaning up data contamination generated during library preparation and the subsequent steps.
Sign Up to like & getrecommendations! Published in 2021 at "Molecular biology reports"
DOI: 10.1007/s11033-021-06884-y
Abstract: BACKGROUND High-throughput sequencing involves library preparation and amplification steps, which may induce contamination across samples or between samples and the environment. METHODS We tested the effect of applying an inline-index strategy, in which DNA indices… read more here.
Keywords: contamination; data contamination; inline index; library preparation ... See more keywords
Testing a series of modifications on genomic library preparation methods for ancient or degraded DNA.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2021.114193
Abstract: Technological advancements have revolutionized ancient and degraded DNA analysis, moving the field to the Next Generation Sequencing era. One of the advancements, the ancient DNA-oriented high-throughput library preparation methods, enabled the sequencing of more endogenous… read more here.
Keywords: ancient degraded; degraded dna; preparation methods; library preparation ... See more keywords
14. A FULLY AUTOMATED INSTRUMENT FOR PREIMPLANTATION GENETIC TESTING (PGT-A) LIBRARY PREPARATION
Sign Up to like & getrecommendations! Published in 2019 at "Reproductive BioMedicine Online"
DOI: 10.1016/j.rbmo.2019.04.067
Abstract: Introduction Technology used in preimplantation genetic testing for aneuploidy (PGT-A) has rapidly evolved since its introduction in 1990. The most commonly used methods use Next Generation Sequencing (NGS) for complete genomic analysis. One example is… read more here.
Keywords: preparation; seq kit; dna; library preparation ... See more keywords
Automation enables high-throughput and reproducible single-cell transcriptomics library preparation.
Sign Up to like & getrecommendations! Published in 2021 at "SLAS technology"
DOI: 10.1016/j.slast.2021.10.018
Abstract: Next-generation sequencing (NGS) has revolutionized genomics, decreasing sequencing costs and allowing researchers to draw correlations between diseases and DNA or RNA changes. Technical advances have enabled the analysis of RNA expression changes between single cells… read more here.
Keywords: single cell; library preparation; cell; scrna seq ... See more keywords
PAR-CLIP and streamlined small RNA cDNA library preparation protocol for the identification of RNA binding protein target sites.
Sign Up to like & getrecommendations! Published in 2017 at "Methods"
DOI: 10.1016/j.ymeth.2016.11.009
Abstract: The study of protein-RNA interactions is critical for our understanding of cellular processes and regulatory circuits controlled by RNA binding proteins (RBPs). Recent next generation sequencing-based approaches significantly promoted our understanding of RNA biology and… read more here.
Keywords: par clip; library preparation; small rna; rna ... See more keywords
Microfluidic platform for next-generation sequencing library preparation with low-input samples.
Sign Up to like & getrecommendations! Published in 2020 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.9b04086
Abstract: Advances in Next Generation Sequencing (NGS) have made available a wealth of information that had previously been inaccessible to researchers and clinicians. NGS has been applied to understand genomic, transcriptomic, and epigenomic changes and gained… read more here.
Keywords: library preparation; input; low input; next generation ... See more keywords
CUTseq is a versatile method for preparing multiplexed DNA sequencing libraries from low-input samples
Sign Up to like & getrecommendations! Published in 2019 at "Nature Communications"
DOI: 10.1038/s41467-019-12570-2
Abstract: Current multiplexing strategies for massively parallel sequencing of genomic DNA mainly rely on library indexing in the final steps of library preparation. This procedure is costly and time-consuming, because a library must be generated separately… read more here.
Keywords: cutseq versatile; dna; library preparation; method ... See more keywords
Evaluation of a high-throughput, cost-effective Illumina library preparation kit
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-94911-0
Abstract: Library preparation for high-throughput sequencing applications is a critical step in producing representative, unbiased sequencing data. The iGenomX Riptide High Throughput Rapid Library Prep Kit purports to provide high-quality sequencing data with lower costs compared… read more here.
Keywords: illumina library; library preparation; kit; high throughput ... See more keywords