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   Articles with "lifetime imaging" as a keyword



Delineation of gastrointestinal tumors biopsies using a fluorescence lifetime imaging optical fiber probe.

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recommendations! Published in 2024 at "Journal of biophotonics"

DOI: 10.1002/jbio.202400122

Abstract: Autofluorescence spectroscopy has emerged in recent years as a powerful tool to report label‐free contrast between normal and diseased tissues, both in vivo and ex‐vivo. We report the application of an instrument employing an optical… read more here.

Keywords: lifetime imaging; optical fiber; fiber probe; lifetime ... See more keywords

Detection sensitivity of fluorescence lifetime imaging ophthalmoscopy for laser-induced selective damage of retinal pigment epithelium

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recommendations! Published in 2024 at "Graefe's Archive for Clinical and Experimental Ophthalmology"

DOI: 10.1007/s00417-024-06449-2

Abstract: To investigate the sensitivity of fluorescence lifetime imaging ophthalmoscopy (FLIO) to detect retinal laser spots by comparative analysis with other imaging modalities. A diode laser with a wavelength of 514 nm was applied with pulse… read more here.

Keywords: laser; fluorescence lifetime; lifetime imaging; laser spots ... See more keywords

Two-photon excited photoluminescence lifetime imaging studies on individual gelatin-coated gold nanorods

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recommendations! Published in 2021 at "Journal of Molecular Structure"

DOI: 10.1016/j.molstruc.2021.130785

Abstract: ABSTRACT The intriguing intrinsic photoluminescence (PL) properties of gold nanorods (AuNRs) have been intensively studied over the last years, not only in the linear but also in the non-linear optics regime, boosting their applicability in… read more here.

Keywords: gelatin; two photon; gold nanorods; lifetime ... See more keywords

Fine Tuning of Multiphoton AIE Emission Behavior, Organelle Targeting, and Fluorescence Lifetime Imaging of Terpyridine Derivatives by Alkyl Chain Engineering.

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recommendations! Published in 2022 at "Analytical chemistry"

DOI: 10.1021/acs.analchem.1c05052

Abstract: In this work, a series of multiphoton terpyridine agents (ZA, ZA-Mex, and ZA-Hex) for fluorescence lifetime imaging microscopy (FLIM) are designed and synthesized. The results from photophysical property research reveal that ZA-Hex, as an N-hexylated… read more here.

Keywords: aie; fluorescence; multiphoton; lifetime imaging ... See more keywords

Recovery Mechanism of Endoplasmic Reticulum Revealed by Fluorescence Lifetime Imaging in Live Cells.

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recommendations! Published in 2022 at "Analytical chemistry"

DOI: 10.1021/acs.analchem.2c00216

Abstract: Endoplasmic reticulum (ER) is an important organelle of a membranous tubule network in cells for the synthesis, assembly, and modification of peptides, proteins, and enzymes. Autophagy and destruction of ER commonly occur during normal cellular… read more here.

Keywords: endoplasmic reticulum; fluorescence lifetime; live cells; lifetime ... See more keywords

Quantifying Antibody Binding Kinetics on Fixed Cells and Tissues via Fluorescence Lifetime Imaging.

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recommendations! Published in 2022 at "Analytical chemistry"

DOI: 10.1021/acs.analchem.2c01076

Abstract: We present a method for monitoring spatially localized antigen-antibody binding events on physiologically relevant substrates (cell and tissue sections) using fluorescence lifetime imaging. Specifically, we use the difference between the fluorescence decay times of fluorescently… read more here.

Keywords: binding kinetics; fluorescence lifetime; antibody binding; lifetime imaging ... See more keywords

Measuring the pH of Acidic Vesicles in Live Cells with an Optimized Fluorescence Lifetime Imaging Probe.

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recommendations! Published in 2022 at "Analytical chemistry"

DOI: 10.1021/acs.analchem.2c01840

Abstract: Acidification of intracellular vesicles, such as endosomes and lysosomes, is a key pathway for regulating the function of internal proteins. Most conventional methods of measuring pH are not satisfactory for quantifying the pH inside these… read more here.

Keywords: fluorescence lifetime; lifetime imaging; fluorescence; acidic vesicles ... See more keywords

In Situ Confocal Fluorescence Lifetime Imaging of Nanopore Electrode Arrays with Redox Active Fluorogenic Amplex Red.

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recommendations! Published in 2022 at "Analytical chemistry"

DOI: 10.1021/acs.analchem.2c03742

Abstract: Direct imaging of electrochemical processes on electrode surfaces is a central part of understanding spatially heterogeneous electrochemical processes on the surfaces. Herein, we report a strategy for the spatially resolved imaging of local faradaic processes… read more here.

Keywords: lifetime imaging; fluorescence lifetime; amplex red; nanopore electrode ... See more keywords

Tailoring the pKa of Fluorescence Lifetime Imaging Probes to Visualize Aggrephagy and Resolve Its Microenvironmental Viscosity.

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recommendations! Published in 2024 at "Analytical chemistry"

DOI: 10.1021/acs.analchem.4c02065

Abstract: Aggrephagy describes lysosomal transport and degradation of protein aggregates via cellular macroautophagy, a key mechanism to prevent neurodegenerative diseases. Here, we develop a dual-probe method to visualize the aggrephagy process and resolve its viscosity heterogeneity… read more here.

Keywords: fluorescence lifetime; lifetime imaging; fluorescence; visualize aggrephagy ... See more keywords

Multiplexing and Sensing with Fluorescence Lifetime Imaging Microscopy Empowered by Phasor U-Net.

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recommendations! Published in 2025 at "Analytical chemistry"

DOI: 10.1021/acs.analchem.5c02028

Abstract: Fluorescence lifetime imaging microscopy (FLIM) has been widely used as an essential multiplexing and sensing tool in frontier fields such as materials science and life sciences. However, the accuracy of lifetime estimation is compromised by… read more here.

Keywords: fluorescence lifetime; lifetime imaging; imaging microscopy; phasor net ... See more keywords

Exploring the Subcellular Localization and Degradation of Spherical Nucleic Acids Using Fluorescence Lifetime Imaging Microscopy

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recommendations! Published in 2025 at "ACS Nano"

DOI: 10.1021/acsnano.5c00177

Abstract: Spherical nucleic acids (SNAs) are a powerful class of nucleic acids with broad applications that span from diagnostic sensors to nanoflares and gene therapeutic agents. SNAs accomplish these varied tasks by taking advantage of the… read more here.

Keywords: fluorescence lifetime; spherical nucleic; degradation; lifetime imaging ... See more keywords