Two-photon excited photoluminescence lifetime imaging studies on individual gelatin-coated gold nanorods
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Molecular Structure"
DOI: 10.1016/j.molstruc.2021.130785
Abstract: ABSTRACT The intriguing intrinsic photoluminescence (PL) properties of gold nanorods (AuNRs) have been intensively studied over the last years, not only in the linear but also in the non-linear optics regime, boosting their applicability in… read more here.
Keywords: gelatin; two photon; gold nanorods; lifetime ... See more keywords
Fine Tuning of Multiphoton AIE Emission Behavior, Organelle Targeting, and Fluorescence Lifetime Imaging of Terpyridine Derivatives by Alkyl Chain Engineering.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.1c05052
Abstract: In this work, a series of multiphoton terpyridine agents (ZA, ZA-Mex, and ZA-Hex) for fluorescence lifetime imaging microscopy (FLIM) are designed and synthesized. The results from photophysical property research reveal that ZA-Hex, as an N-hexylated… read more here.
Keywords: aie; fluorescence; multiphoton; lifetime imaging ... See more keywords
Recovery Mechanism of Endoplasmic Reticulum Revealed by Fluorescence Lifetime Imaging in Live Cells.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c00216
Abstract: Endoplasmic reticulum (ER) is an important organelle of a membranous tubule network in cells for the synthesis, assembly, and modification of peptides, proteins, and enzymes. Autophagy and destruction of ER commonly occur during normal cellular… read more here.
Keywords: endoplasmic reticulum; fluorescence lifetime; live cells; lifetime ... See more keywords
Quantifying Antibody Binding Kinetics on Fixed Cells and Tissues via Fluorescence Lifetime Imaging.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c01076
Abstract: We present a method for monitoring spatially localized antigen-antibody binding events on physiologically relevant substrates (cell and tissue sections) using fluorescence lifetime imaging. Specifically, we use the difference between the fluorescence decay times of fluorescently… read more here.
Keywords: binding kinetics; fluorescence lifetime; antibody binding; lifetime imaging ... See more keywords
Measuring the pH of Acidic Vesicles in Live Cells with an Optimized Fluorescence Lifetime Imaging Probe.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c01840
Abstract: Acidification of intracellular vesicles, such as endosomes and lysosomes, is a key pathway for regulating the function of internal proteins. Most conventional methods of measuring pH are not satisfactory for quantifying the pH inside these… read more here.
Keywords: fluorescence lifetime; lifetime imaging; fluorescence; acidic vesicles ... See more keywords
In Situ Confocal Fluorescence Lifetime Imaging of Nanopore Electrode Arrays with Redox Active Fluorogenic Amplex Red.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c03742
Abstract: Direct imaging of electrochemical processes on electrode surfaces is a central part of understanding spatially heterogeneous electrochemical processes on the surfaces. Herein, we report a strategy for the spatially resolved imaging of local faradaic processes… read more here.
Keywords: lifetime imaging; fluorescence lifetime; amplex red; nanopore electrode ... See more keywords
Quinolinium-Based Fluorescent Probes for Dynamic pH Monitoring in Aqueous Media at High pH Using Fluorescence Lifetime Imaging
Sign Up to like & getrecommendations! Published in 2023 at "ACS Sensors"
DOI: 10.1021/acssensors.3c00316
Abstract: Spatiotemporal pH imaging using fluorescence lifetime imaging microscopy (FLIM) is an excellent technique for investigating dynamic (electro)chemical processes. However, probes that are responsive at high pH values are not available. Here, we describe the development… read more here.
Keywords: quinolinium; fluorescence lifetime; lifetime imaging; using fluorescence ... See more keywords
Monitoring intracellular nanomolar calcium using fluorescence lifetime imaging.
Sign Up to like & getrecommendations! Published in 2018 at "Nature protocols"
DOI: 10.1038/nprot.2017.154
Abstract: Nanomolar-range fluctuations of intracellular [Ca2+] are critical for brain cell function but remain difficult to measure. We have advanced a microscopy technique to monitor intracellular [Ca2+] in individual cells in acute brain slices (also applicable… read more here.
Keywords: lifetime imaging; fluorescence lifetime; cell; using fluorescence ... See more keywords
Fluorescence lifetime imaging with a megapixel SPAD camera and neural network lifetime estimation
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-77737-0
Abstract: Fluorescence lifetime imaging microscopy (FLIM) is a key technology that provides direct insight into cell metabolism, cell dynamics and protein activity. However, determining the lifetimes of different fluorescent proteins requires the detection of a relatively… read more here.
Keywords: lifetime estimation; fluorescence; lifetime imaging; fluorescence lifetime ... See more keywords
Simple phasor-based deep neural network for fluorescence lifetime imaging microscopy
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-03060-x
Abstract: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to probe the molecular environment of fluorophores. The analysis of FLIM images is usually performed with time consuming fitting methods. For accelerating this analysis, sophisticated deep… read more here.
Keywords: fluorescence; microscopy; network; lifetime imaging ... See more keywords
Lifetime imaging of GFP at CoxVIIIa reports respiratory supercomplex assembly in live cells
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/srep46055
Abstract: The assembly of respiratory complexes into macromolecular supercomplexes is currently a hot topic, especially in the context of newly available structural details. However, most work to date has been done with purified detergent-solubilized material and… read more here.
Keywords: respiratory supercomplex; coxviiia; lifetime imaging; gfp coxviiia ... See more keywords