Ligand fishing with cellular membrane-coated cellulose filter paper: a new method for screening of potential active compounds from natural products
Sign Up to like & getrecommendations! Published in 2019 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-019-01662-z
Abstract: AbstractLigand fishing is a widely used approach for screening active compounds from natural products. Recently, cell membrane (CM) as affinity ligand has been applied in ligand fishing, including cell membrane chromatography (CMC) and CM-coated magnetic… read more here.
Keywords: natural products; ligand fishing; active compounds; filter paper ... See more keywords
High-resolution inhibition profiling and ligand fishing for screening of nucleoside hydrolase ligands in Moringa oleifera Lamarck.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of pharmaceutical and biomedical analysis"
DOI: 10.1016/j.jpba.2022.114614
Abstract: In Leishmania donovani, the causative protozoan of visceral leishmaniasis, nucleoside hydrolase enzyme (NH) is fundamental for the biosynthesis of its DNA and RNA. Therefore, LdNH is considered a potential target for the development of new… read more here.
Keywords: high resolution; nucleoside hydrolase; ligand fishing; inhibition ... See more keywords
Combined magnetic ligand fishing and high-resolution inhibition profiling for identification of α-glucosidase inhibitory ligands: A new screening approach based on complementary inhibition and affinity profiles.
Sign Up to like & getrecommendations! Published in 2019 at "Talanta"
DOI: 10.1016/j.talanta.2019.03.047
Abstract: Plants are well-recognized sources of inhibitors for α-glucosidase - a key target enzyme for management of type 2 diabetes. Recently, two advanced bioactivity-profiling techniques, i.e., ligand fishing and high-resolution inhibition profiling, have shown great promises… read more here.
Keywords: resolution inhibition; high resolution; fishing high; inhibition profiling ... See more keywords
Immobilized enzyme for screening and identification of anti-diabetic components from natural products by ligand fishing
Sign Up to like & getrecommendations! Published in 2022 at "Critical Reviews in Biotechnology"
DOI: 10.1080/07388551.2021.2025034
Abstract: Abstract Diabetes is a chronic metabolic disease caused by insufficient insulin secretion and insulin resistance. Natural product is one of the most important resources for anti-diabetic drug. However, due to the extremely complex composition, this… read more here.
Keywords: natural products; anti diabetic; immobilized enzyme; enzyme screening ... See more keywords
Efficient screening of pancreatic lipase inhibitors from cod meat hydrolysate through ligand fishing strategy
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Nutrition"
DOI: 10.3389/fnut.2022.969558
Abstract: Obesity has become an increasingly serious public health problem. Pancreatic lipase (PL) is identified as a ideal target for the prevention and treatment of obesity. Orlistat, the only approved PL inhibitor (PLI), is a powerful… read more here.
Keywords: meat hydrolysate; cod meat; hydrolysate; pancreatic lipase ... See more keywords