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   Articles with "ligation" as a keyword



How chromosome topologies get their shape: views from proximity ligation and microscopy methods

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recommendations! Published in 2020 at "FEBS Letters"

DOI: 10.1002/1873-3468.13961

Abstract: The 3D organization of our genome is an important determinant for the transcriptional output of a gene in (patho)physiological contexts. The spatial organization of linear chromosomes within nucleus is dominantly inferred using two distinct approaches,… read more here.

Keywords: proximity ligation; microscopy; ligation; dna fish ... See more keywords

Modular Ligation Extension of Guide RNA Operons (LEGO) for Multiplexed dCas9 Regulation of Metabolic Pathways in Saccharomyces cerevisiae.

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recommendations! Published in 2018 at "Biotechnology journal"

DOI: 10.1002/biot.201700582

Abstract: Metabolic engineering typically utilizes a suboptimal step-wise gene target optimization approach to parse a highly connected and regulated cellular metabolism. While the endonuclease-null CRISPR/Cas system has enabled gene expression perturbations without genetic modification, it has… read more here.

Keywords: sgrna; operons lego; ligation; approach ... See more keywords

Native Chemical Ligation Directed by Photocleavable Peptide Nucleic Acid (PNA) Templates

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recommendations! Published in 2017 at "ChemBioChem"

DOI: 10.1002/cbic.201700487

Abstract: A novel peptide–peptide ligation strategy is introduced that has the potential to provide peptide libraries of linearly or branched coupled fragments and will be suited to introduce simultaneous protein modifications at different ligation sites. Ligation… read more here.

Keywords: pna; peptide nucleic; ligation; nucleic acid ... See more keywords

Tips and pitfalls in direct ligation of large spontaneous splenorenal shunt during liver transplantation

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recommendations! Published in 2017 at "Liver Transplantation"

DOI: 10.1002/lt.24783

Abstract: Patients with large spontaneous splenorenal shunts (SRSs) prove challenging during liver transplantation (LT), regardless of organizing portal vein (PV) thrombosis. Here, we detail the clinical outcomes of 26 patients who underwent direct ligation of large… read more here.

Keywords: liver transplantation; ligation large; ligation; direct ligation ... See more keywords

Lung ultrasound score predicts patent ductus arteriosus ligation among neonates ≤25 weeks.

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recommendations! Published in 2023 at "Pediatric pulmonology"

DOI: 10.1002/ppul.26531

Abstract: BACKGROUND This prospective study aimed to investigate whether lung ultrasound score (LUSs) can predict the patent ductus arteriosus (PDA) ligation. METHODS Preterm infants ≤25 weeks of gestational age (GA) were enrolled. A lung ultrasound was performed on the… read more here.

Keywords: patent ductus; ligation; ultrasound score; lung ultrasound ... See more keywords

Highly Precise Protein Semisynthesis through Ligation-Desulfurization Chemistry in Combination with Phenacyl Protection of Native Cysteines.

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recommendations! Published in 2020 at "Methods in molecular biology"

DOI: 10.1007/978-1-0716-0434-2_17

Abstract: Semisynthesis of proteins via expressed protein ligation is a powerful tool to furnish full-length proteins carrying site-specific (posttranslational) modifications. The development of various β-mercapto amino acid building blocks coupled with ligation-desulfurization chemistry enabled further advances… read more here.

Keywords: protein; chemistry; ligation; native cysteines ... See more keywords

The Use of Methylation-Sensitive Multiplex Ligation-Dependent Probe Amplification for Quantification of Imprinted Methylation.

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recommendations! Published in 2018 at "Methods in molecular biology"

DOI: 10.1007/978-1-4939-7768-0_6

Abstract: Imprinting disorders are a group of congenital diseases that can result from multiple mechanisms affecting imprinted gene dosage including cytogenetic aberration and epigenetic anomalies. Quantification of CpG methylation and correct copy-number calling is required for… read more here.

Keywords: methylation; probe; ligation; sensitive multiplex ... See more keywords

DNA Damage In Situ Ligation Followed by Proximity Ligation Assay (DI-PLA).

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recommendations! Published in 2019 at "Methods in molecular biology"

DOI: 10.1007/978-1-4939-8931-7_2

Abstract: Cells have evolved DNA repair mechanisms to maintain their genetic information unaltered and a DNA damage response pathway that coordinates DNA repair with several cellular events. Despite a clear role for DNA damage in the… read more here.

Keywords: dna damage; dna; pla; ligation ... See more keywords

Retrospective analysis of the perioperative outcome in living donor kidney transplantation with multiple renal arteries: does accessory vessel ligation affect the outcome?

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recommendations! Published in 2024 at "World Journal of Urology"

DOI: 10.1007/s00345-024-04883-9

Abstract: Purpose Accurate surgical reconstruction of arterial vascular supply is a crucial part of living kidney transplantation (LDKT). The presence of multiple renal arteries (MRA) in grafts can be challenging. In the present study, we investigated… read more here.

Keywords: kidney transplantation; outcome; ligation; renal arteries ... See more keywords

Acute and long-term outcomes of epicardial left atrial appendage ligation with the second-generation LARIAT device: a high-volume electrophysiology center experience

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recommendations! Published in 2018 at "Clinical Research in Cardiology"

DOI: 10.1007/s00392-018-1288-1

Abstract: BackgroundEpicardial left atrial appendage (LAA) ligation may be an alternative to oral anticoagulation management and to endocardial LAA closure devices. We report long-term results after LAA ligation with the second-generation LARIAT device.MethodsRetrospective study was performed… read more here.

Keywords: laa closure; ligation; laa ligation; generation lariat ... See more keywords

Modified hysterectomy for placenta increta and percreta: modifications of what?

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recommendations! Published in 2019 at "Archives of Gynecology and Obstetrics"

DOI: 10.1007/s00404-019-05114-y

Abstract: Hussein et al. claimed that four technical modifications were made in cesarean hysterectomy for placenta accreta spectrum (PAS) disorders [1]: (1) the retroperitoneum was opened to laterally mobilize the ureter, (2) cutting and ligating vessels… read more here.

Keywords: bladder; ligation; bladder separation; separation ... See more keywords