How chromosome topologies get their shape: views from proximity ligation and microscopy methods
Sign Up to like & getrecommendations! Published in 2020 at "FEBS Letters"
DOI: 10.1002/1873-3468.13961
Abstract: The 3D organization of our genome is an important determinant for the transcriptional output of a gene in (patho)physiological contexts. The spatial organization of linear chromosomes within nucleus is dominantly inferred using two distinct approaches,… read more here.
Keywords: proximity ligation; microscopy; ligation; dna fish ... See more keywords
Modular Ligation Extension of Guide RNA Operons (LEGO) for Multiplexed dCas9 Regulation of Metabolic Pathways in Saccharomyces cerevisiae.
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology journal"
DOI: 10.1002/biot.201700582
Abstract: Metabolic engineering typically utilizes a suboptimal step-wise gene target optimization approach to parse a highly connected and regulated cellular metabolism. While the endonuclease-null CRISPR/Cas system has enabled gene expression perturbations without genetic modification, it has… read more here.
Keywords: sgrna; operons lego; ligation; approach ... See more keywords
Native Chemical Ligation Directed by Photocleavable Peptide Nucleic Acid (PNA) Templates
Sign Up to like & getrecommendations! Published in 2017 at "ChemBioChem"
DOI: 10.1002/cbic.201700487
Abstract: A novel peptide–peptide ligation strategy is introduced that has the potential to provide peptide libraries of linearly or branched coupled fragments and will be suited to introduce simultaneous protein modifications at different ligation sites. Ligation… read more here.
Keywords: pna; peptide nucleic; ligation; nucleic acid ... See more keywords
Tips and pitfalls in direct ligation of large spontaneous splenorenal shunt during liver transplantation
Sign Up to like & getrecommendations! Published in 2017 at "Liver Transplantation"
DOI: 10.1002/lt.24783
Abstract: Patients with large spontaneous splenorenal shunts (SRSs) prove challenging during liver transplantation (LT), regardless of organizing portal vein (PV) thrombosis. Here, we detail the clinical outcomes of 26 patients who underwent direct ligation of large… read more here.
Keywords: liver transplantation; ligation large; ligation; direct ligation ... See more keywords
Lung ultrasound score predicts patent ductus arteriosus ligation among neonates ≤25 weeks.
Sign Up to like & getrecommendations! Published in 2023 at "Pediatric pulmonology"
DOI: 10.1002/ppul.26531
Abstract: BACKGROUND This prospective study aimed to investigate whether lung ultrasound score (LUSs) can predict the patent ductus arteriosus (PDA) ligation. METHODS Preterm infants ≤25 weeks of gestational age (GA) were enrolled. A lung ultrasound was performed on the… read more here.
Keywords: patent ductus; ligation; ultrasound score; lung ultrasound ... See more keywords
Highly Precise Protein Semisynthesis through Ligation-Desulfurization Chemistry in Combination with Phenacyl Protection of Native Cysteines.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0434-2_17
Abstract: Semisynthesis of proteins via expressed protein ligation is a powerful tool to furnish full-length proteins carrying site-specific (posttranslational) modifications. The development of various β-mercapto amino acid building blocks coupled with ligation-desulfurization chemistry enabled further advances… read more here.
Keywords: protein; chemistry; ligation; native cysteines ... See more keywords
The Use of Methylation-Sensitive Multiplex Ligation-Dependent Probe Amplification for Quantification of Imprinted Methylation.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7768-0_6
Abstract: Imprinting disorders are a group of congenital diseases that can result from multiple mechanisms affecting imprinted gene dosage including cytogenetic aberration and epigenetic anomalies. Quantification of CpG methylation and correct copy-number calling is required for… read more here.
Keywords: methylation; probe; ligation; sensitive multiplex ... See more keywords
DNA Damage In Situ Ligation Followed by Proximity Ligation Assay (DI-PLA).
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8931-7_2
Abstract: Cells have evolved DNA repair mechanisms to maintain their genetic information unaltered and a DNA damage response pathway that coordinates DNA repair with several cellular events. Despite a clear role for DNA damage in the… read more here.
Keywords: dna damage; dna; pla; ligation ... See more keywords
Acute and long-term outcomes of epicardial left atrial appendage ligation with the second-generation LARIAT device: a high-volume electrophysiology center experience
Sign Up to like & getrecommendations! Published in 2018 at "Clinical Research in Cardiology"
DOI: 10.1007/s00392-018-1288-1
Abstract: BackgroundEpicardial left atrial appendage (LAA) ligation may be an alternative to oral anticoagulation management and to endocardial LAA closure devices. We report long-term results after LAA ligation with the second-generation LARIAT device.MethodsRetrospective study was performed… read more here.
Keywords: laa closure; ligation; laa ligation; generation lariat ... See more keywords
Modified hysterectomy for placenta increta and percreta: modifications of what?
Sign Up to like & getrecommendations! Published in 2019 at "Archives of Gynecology and Obstetrics"
DOI: 10.1007/s00404-019-05114-y
Abstract: Hussein et al. claimed that four technical modifications were made in cesarean hysterectomy for placenta accreta spectrum (PAS) disorders [1]: (1) the retroperitoneum was opened to laterally mobilize the ureter, (2) cutting and ligating vessels… read more here.
Keywords: bladder; ligation; bladder separation; separation ... See more keywords
Laparoscopic complete mesocolic excision with true central vascular ligation for right-sided colon cancer
Sign Up to like & getrecommendations! Published in 2020 at "Surgical Endoscopy"
DOI: 10.1007/s00464-020-07867-z
Abstract: Complete mesocolic excision (CME) is known to be effective for colon cancer. However, in right-sided colon cancer, central vascular ligation (CVL) is not easy to perform. In particular, in patients in whom the superior mesenteric… read more here.
Keywords: right sided; ligation; sided colon; colon ... See more keywords