Proximity Ligation Assay for Detecting Protein‐Protein Interactions and Protein Modifications in Cells and Tissues in Situ
Sign Up to like & getrecommendations! Published in 2020 at "Current Protocols in Cell Biology"
DOI: 10.1002/cpcb.115
Abstract: Biochemical methods can reveal stable protein‐protein interactions occurring within cells, but the ability to observe transient events and to visualize the subcellular localization of protein‐protein interactions in cells and tissues in situ provides important additional… read more here.
Keywords: protein; proximity ligation; protein interactions; ligation assay ... See more keywords
Proximity Ligation Assay (PLA) to Evaluate DISC and MISC Composition.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6780-3_5
Abstract: Distribution of the death receptor CD95 into lipid rafts (aggregation) and/or its internalization may contribute to the implementation of the apoptotic signal at the detriment of the non-apoptotic signaling pathway [1-6]. Also CD95 can form… read more here.
Keywords: assay pla; pla; proximity ligation; ligation assay ... See more keywords
Proximity ligation assay: an ultrasensitive method for protein quantification and its applications in pathogen detection.
Sign Up to like & getrecommendations! Published in 2021 at "Applied microbiology and biotechnology"
DOI: 10.1007/s00253-020-11049-1
Abstract: It is of great significance to establish sensitive and accurate pathogen detection methods, considering the continuous emergence or re-emergence of infectious diseases seriously influences the safety of human and animals. Proximity ligation assay (PLA) is… read more here.
Keywords: protein; detection; proximity ligation; ligation assay ... See more keywords
Proximity ligation assay induced hairpin to DNAzyme structure switching for entropy-driven amplified detection of thrombin.
Sign Up to like & getrecommendations! Published in 2019 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2019.03.007
Abstract: A proximity ligation assay (PLA) induced hairpin to DNAzyme structure switching strategy has been described for entropy-driven amplified detection of thrombin. The enzyme-strand (E-DNA) and substrate-strand (S-DNA) of DNAzyme are locked in hairpins structure, and… read more here.
Keywords: dnazyme; proximity ligation; entropy driven; structure ... See more keywords
Multiplex Single-Molecule DNA Barcoding Using an Oligonucleotide Ligation Assay.
Sign Up to like & getrecommendations! Published in 2018 at "Biophysical journal"
DOI: 10.1016/j.bpj.2018.08.013
Abstract: Detection of specific nucleic acid sequences is invaluable in biological studies such as genetic disease diagnostics and genome profiling. Here, we developed a highly sensitive and specific detection method that combines an advanced oligonucleotide ligation… read more here.
Keywords: single molecule; oligonucleotide ligation; dna; ligation ... See more keywords
Proximity ligation assay to study TSH receptor homodimerization and crosstalk with IGF-1 receptors in human thyroid cells
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Endocrinology"
DOI: 10.3389/fendo.2022.989626
Abstract: Proximity ligation assay (PLA) is a methodology that permits detection of protein-protein closeness, that is, proteins that are within 40 nanometers of each other, in cells or tissues at endogenous protein levels or after exogenous… read more here.
Keywords: receptor homodimerization; ligation assay; proximity ligation; tsh receptor ... See more keywords