Magnetic Bead Processing Enables Sensitive Ligation-Based Detection of HIV Drug Resistance Mutations.
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DOI: 10.1021/acs.analchem.1c05040
Abstract: HIV develops single nucleotide polymorphisms (SNPs), some of which lead to drug resistance mutations (DRMs) that prevent therapeutic viral suppression. Genomic sequencing enables healthcare professionals to select effective combination antiretroviral therapy (ART) to achieve and… read more here.
Keywords: ligation based; processing; ligation; resistance mutations ... See more keywords
Ligation‐based assay for variant typing without sequencing: Application to SARS‐CoV‐2 variants of concern
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DOI: 10.1111/irv.13083
Abstract: COVID‐19 prevalence has remained high throughout the pandemic with intermittent surges, due largely to the emergence of genetic variants, demonstrating the need for more accessible sequencing technologies for strain typing. read more here.
Keywords: ligation based; variant typing; assay variant; typing without ... See more keywords
Excess primer degradation by Exo I improves the preparation of 3' cDNA ligation-based sequencing libraries.
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DOI: 10.2144/btn-2018-0178
Abstract: RNA sequencing library construction using single-stranded ligation of a DNA adapter to 3' ends of cDNAs often produces primer-adapter byproducts, which compete with cDNA-adapter ligation products during library amplification and, therefore, reduces the number of… read more here.
Keywords: cdna ligation; based sequencing; ligation; ligation based ... See more keywords