Multi-contact 4C: long-molecule sequencing of complex proximity ligation products to uncover local cooperative and competitive chromatin topologies
Sign Up to like & getrecommendations! Published in 2020 at "Nature Protocols"
DOI: 10.1038/s41596-019-0242-7
Abstract: We present the experimental protocol and data analysis toolbox for multi-contact 4C (MC-4C), a new proximity ligation method tailored to study the higher-order chromatin contact patterns of selected genomic sites. Conventional chromatin conformation capture (3C)… read more here.
Keywords: proximity ligation; chromatin; analysis; ligation products ... See more keywords
Polymerase Chain Reaction (PCR) Detection of 3C Ligation Products Present in 3C, ChIP-Loop, and Control Libraries: Library Titration and Interaction Frequency Analysis.
Sign Up to like & getrecommendations! Published in 2018 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot097873
Abstract: In all 3C-based assays, the aim is to measure the frequency with which two loci interact within cells. This is achieved by determining the relative abundance of ligation products formed during the 3C protocol that… read more here.
Keywords: interaction; ligation products; ligation; pcr detection ... See more keywords