A bi-terminal protein ligation strategy to probe chromatin structure during DNA damage† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c8sc00681d
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DOI: 10.1039/c8sc00681d
Abstract: A convenient method to bi-terminally modify proteins using recombinant masking groups reveals that H2A.X ubiquitylation opens chromatin during DNA repair. read more here.
Keywords: protein ligation; dna; ligation strategy; terminal protein ... See more keywords
An in-library ligation strategy and its application in CRISPR/Cas9 screening of high-order gRNA combinations
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac458
Abstract: Abstract Simultaneous targeting multiple genes is a big advantage of CRISPR (clustered regularly interspaced short palindromic repeats) genome editing but challenging to achieve in CRISPR screening. The crosstalk among genes or gene products is a… read more here.
Keywords: high order; library ligation; crispr; ligation strategy ... See more keywords