Light-Inducible CRISPR Labeling.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0755-8_9
Abstract: CRISPR labeling is a powerful technique to study the chromatin architecture in live cells. In CRISPR labeling, a catalytically dead CRISPR-Cas9 mutant is employed as programmable DNA-binding domain to recruit fluorescent proteins to selected genomic… read more here.
Keywords: light inducible; crispr; microscopy; inducible crispr ... See more keywords
Binding of pigments to the cyanobacterial high-light-inducible protein HliC
Sign Up to like & getrecommendations! Published in 2017 at "Photosynthesis Research"
DOI: 10.1007/s11120-017-0475-7
Abstract: Cyanobacteria possess a family of one-helix high-light-inducible proteins (HLIPs) that are widely viewed as ancestors of the light-harvesting antenna of plants and algae. HLIPs are essential for viability under various stress conditions, although their exact… read more here.
Keywords: protein; light inducible; high light; pigments cyanobacterial ... See more keywords
High-light-inducible proteins HliA and HliB: pigment binding and protein-protein interactions.
Sign Up to like & getrecommendations! Published in 2022 at "Photosynthesis research"
DOI: 10.1007/s11120-022-00904-z
Abstract: High-light-inducible proteins (Hlips) are single-helix transmembrane proteins that are essential for the survival of cyanobacteria under stress conditions. The model cyanobacterium Synechocystis sp. PCC 6803 contains four Hlip isoforms (HliA-D) that associate with Photosystem II… read more here.
Keywords: pigment binding; high light; light inducible; inducible proteins ... See more keywords
Light-inducible deformation of mitochondria in live cells.
Sign Up to like & getrecommendations! Published in 2021 at "Cell chemical biology"
DOI: 10.1016/j.chembiol.2021.05.015
Abstract: Mitochondria, the powerhouse of the cell, are dynamic organelles that undergo constant morphological changes. Increasing evidence indicates that mitochondria morphologies and functions can be modulated by mechanical cues. However, the mechano-sensing and -responding properties of… read more here.
Keywords: light inducible; deformation mitochondria; mitochondria live; live cells ... See more keywords
Engineering a Blue Light Inducible SpyTag System (BLISS).
Sign Up to like & getrecommendations! Published in 2021 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.1c03198
Abstract: The SpyCatcher/SpyTag protein conjugation system has recently exploded in popularity due to its fast kinetics and high yield under biologically favorable conditions in both in vitro and intracellular settings. The utility of this system could… read more here.
Keywords: blue light; inducible spytag; light inducible; system ... See more keywords
A Light-Inducible Strain for Genome-Wide Histone Turnover Profiling in Neurospora crassa
Sign Up to like & getrecommendations! Published in 2020 at "Genetics"
DOI: 10.1534/genetics.120.303217
Abstract: In chromatin, nucleosomes are composed of ∼146 bp of DNA wrapped around a histone octamer, and are highly dynamic structures subject to remodeling and exchange. Histone turnover has previously been implicated in various processes including… read more here.
Keywords: light inducible; neurospora crassa; chromatin; histone turnover ... See more keywords
Optogenetic activation of intracellular signaling based on light-inducible protein-protein homo-interactions
Sign Up to like & getrecommendations! Published in 2022 at "Neural Regeneration Research"
DOI: 10.4103/1673-5374.314293
Abstract: Dynamic protein-protein interactions are essential for proper cell functioning. Homo-interaction events—physical interactions between the same type of proteins—represent a pivotal subset of protein-protein interactions that are widely exploited in activating intracellular signaling pathways. Capacities of… read more here.
Keywords: protein; light inducible; intracellular signaling; protein homo ... See more keywords