Development and Applications of Fluorogen/Light-Up RNA Aptamer Pairs for RNA Detection and More.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0712-1_5
Abstract: The central role of RNA in living systems made it highly desirable to have noninvasive and sensitive technologies allowing for imaging the synthesis and the location of these molecules in living cells. This need motivated… read more here.
Keywords: fluorogen light; light rna; rna aptamer; development applications ... See more keywords
Genetically Encoded Light-Up RNA Amplifier Dissecting MicroRNA Activity in Live Cells.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c03643
Abstract: Live cell dissection of microRNA activities is crucial for basic and translational medicine, but current hybridization-based strategies may fail to dissect surrounding-dependent activities. Here, we develop a genetically encoded miRNA-induced light-up RNA amplifier (iLAMP) that… read more here.
Keywords: light rna; live cells; genetically encoded; amplifier dissecting ... See more keywords
Construction of Genetically Encoded Light-Up RNA Aptamers for Label-free and Ultrasensitive Detection of CircRNAs in Cancer Cells and Tissues.
Sign Up to like & getrecommendations! Published in 2023 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.3c01624
Abstract: Circular RNAs (circRNAs) as endogenous non-coding RNAs are characterized by covalently closed circular structures, and they widely exist in mammalian cells. The aberrant expression of circRNAs may result in various diseases. Herein, we demonstrate the… read more here.
Keywords: construction genetically; light rna; rna aptamers; detection ... See more keywords
A novel method for miRNA detection based on target-triggered transcription of a light-up RNA aptamer.
Sign Up to like & getrecommendations! Published in 2022 at "Chemical communications"
DOI: 10.1039/d1cc07095a
Abstract: We herein describe a novel method for miRNA detection based on target-triggered transcription of a light-up RNA aptamer (TTRApt), consequently producing a highly enhanced fluorescence signal through specific binding of a light-up RNA aptamer to… read more here.
Keywords: light rna; rna aptamer; mirna detection; novel method ... See more keywords
Light-Up RNA Aptamers and Their Cognate Fluorogens: From Their Development to Their Applications
Sign Up to like & getrecommendations! Published in 2017 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms19010044
Abstract: An RNA-based fluorogenic module consists of a light-up RNA aptamer able to specifically interact with a fluorogen to form a fluorescent complex. Over the past decade, significant efforts have been devoted to the development of… read more here.
Keywords: rna aptamers; light rna; aptamers cognate; cognate fluorogens ... See more keywords