Light sheet imaging and interactive analysis of the cardiac structure in neonatal mice
Sign Up to like & getrecommendations! Published in 2023 at "Journal of Biophotonics"
DOI: 10.1002/jbio.202200278
Abstract: Light‐sheet microscopy (LSM) enables us to strengthen the understanding of cardiac development, injury, and regeneration in mammalian models. This emerging technique decouples laser illumination and fluorescence detection to investigate cardiac micro‐structure and function with a… read more here.
Keywords: sheet imaging; light sheet; structure; imaging interactive ... See more keywords
Light Sheet Microscopy to Measure Protein Dynamics
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Cellular Physiology"
DOI: 10.1002/jcp.25451
Abstract: Visualizing protein dynamics is the key to a quantitative understanding of molecular mechanisms in biological systems. Recent developments in fluorescent microscopy techniques allow for novel experimental approaches to study stochastic localization, distribution, and movement of… read more here.
Keywords: protein dynamics; microscopy; measure protein; light sheet ... See more keywords
Selectable light‐sheet uniformity using tuned axial scanning
Sign Up to like & getrecommendations! Published in 2017 at "Microscopy Research and Technique"
DOI: 10.1002/jemt.22795
Abstract: Light‐sheet fluorescence microscopy (LSFM) is an optical sectioning technique capable of rapid three‐dimensional (3D) imaging of a wide range of specimens with reduced phototoxicity and superior background rejection. However, traditional light‐sheet generation approaches based on… read more here.
Keywords: microscopy; sheet; light sheet; sheet uniformity ... See more keywords
Light‐sheet based lithography technique for patterning an array of microfluidic channels
Sign Up to like & getrecommendations! Published in 2018 at "Microscopy Research and Technique"
DOI: 10.1002/jemt.22823
Abstract: We propose a Light‐sheet laser interference lithography technique for fabricating periodic microfluidic channels. This technique uses multiple light‐sheet illumination pattern that is generated using a spatial filter at the back‐aperture of the cylindrical lens. Specially… read more here.
Keywords: technique; microfluidic channels; lithography technique; light sheet ... See more keywords
Introduction to the special section on light sheet fluorescence illumination microscopy
Sign Up to like & getrecommendations! Published in 2018 at "Microscopy Research and Technique"
DOI: 10.1002/jemt.23136
Abstract: In the last decade, light sheet fluorescence illumination microscopy (LSFM) became a more and more popular tool for three-dimensional imaging of living organisms and samples. Thanks to the confined excitation volume, this technique provides an… read more here.
Keywords: illumination microscopy; light sheet; biology; microscopy ... See more keywords
Automatic detector synchronization for long‐term imaging using confocal light‐sheet microscopy
Sign Up to like & getrecommendations! Published in 2022 at "Microscopy Research and Technique"
DOI: 10.1002/jemt.24223
Abstract: Light sheet fluorescence microscopy (LSFM) is an important tool in developmental biology. In this microscopy technique confocal line detection is often used to improve image contrast. To this end, the image of the illuminating scanned… read more here.
Keywords: long term; light sheet; laser; microscopy ... See more keywords
Scattering‐Based Light‐Sheet Microscopy for Rapid Cellular Imaging of Fresh Tissue
Sign Up to like & getrecommendations! Published in 2020 at "Lasers in Surgery and Medicine"
DOI: 10.1002/lsm.23361
Abstract: Light‐sheet microscopy (LSM) is a novel imaging technology that has been used for imaging fluorescence contrast in basic life science research. In this paper, we have developed a scattering‐based LSM (sLSM) for rapidly imaging the… read more here.
Keywords: microscopy; scattering based; based light; light sheet ... See more keywords
Quantifiable Intravital Light Sheet Microscopy.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-2051-9_11
Abstract: Live imaging of zebrafish embryos that maintains normal development can be difficult to achieve due to a combination of sample mounting, immobilization, and phototoxicity issues that, once overcome, often still results in image quality sufficiently… read more here.
Keywords: sheet microscopy; microscopy; intravital light; light sheet ... See more keywords
Three-Dimensional Live Imaging of Filamentous Fungi with Light Sheet-Based Fluorescence Microscopy (LSFM).
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6810-7_2
Abstract: We describe a method for the three-dimensional live imaging of filamentous fungi with light sheet-based fluorescence microscopy (LSFM). LSFM provides completely new opportunities to investigate the biology of fungal cells and other microorganisms with high… read more here.
Keywords: three dimensional; microscopy; fungi light; biology ... See more keywords
Live Imaging of Cell Invasion Using a Multicellular Spheroid Model and Light-Sheet Microscopy.
Sign Up to like & getrecommendations! Published in 2017 at "Advances in experimental medicine and biology"
DOI: 10.1007/978-3-319-67358-5_11
Abstract: Three-dimensional cellular assays are becoming increasingly popular as a fundamental tool to bridge the gap between tissue culture systems and in vivo tissue. In particular, spheroids are recognised today as a necessary intermediate model between… read more here.
Keywords: imaging cell; microscopy; model; light sheet ... See more keywords
Light-Sheet Imaging to Elucidate Cardiovascular Injury and Repair
Sign Up to like & getrecommendations! Published in 2018 at "Current Cardiology Reports"
DOI: 10.1007/s11886-018-0979-6
Abstract: Purpose of ReviewReal-time 3-dimensional (3-D) imaging of cardiovascular injury and regeneration remains challenging. We introduced a multi-scale imaging strategy that uses light-sheet illumination to enable applications of cardiovascular injury and repair in models ranging from… read more here.
Keywords: injury repair; sheet imaging; sheet; light sheet ... See more keywords