Identification of two subtilisin‐like serine proteases engaged in the degradation of recombinant proteins in Nicotiana benthamiana
Sign Up to like & getrecommendations! Published in 2020 at "Febs Letters"
DOI: 10.1002/1873-3468.14014
Abstract: The tobacco variant Nicotiana benthamiana has recently emerged as a versatile host for the manufacturing of protein therapeutics, but the fidelity of many recombinant proteins generated in this system is compromised by inadvertent proteolysis. Previous… read more here.
Keywords: serine proteases; subtilisin like; like serine; recombinant proteins ... See more keywords
A snake‐like serine proteinase (PmSnake) activates prophenoloxidase‐activating system in black tiger shrimp Penaeus monodon
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DOI: 10.1016/j.dci.2016.09.016
Abstract: ABSTRACT Clip domain serine proteinases (ClipSPs) play critical roles in the activation of proteolytic cascade in invertebrate immune systems including the prophenoloxidase (proPO) activating system. In this study, we characterized a snake‐like serine protease, namely… read more here.
Keywords: system; shrimp; like serine; serine proteinase ... See more keywords
A trypsin-like serine protease domain of masquerade gene in crayfish Procambarus clarkii could activate prophenoloxidase and inhibit bacterial growth.
Sign Up to like & getrecommendations! Published in 2020 at "Developmental and comparative immunology"
DOI: 10.1016/j.dci.2020.103980
Abstract: Masquerade (Mas) is a secreted trypsin-like serine protease (SPs) and involved in immune response in some arthropods. However, according to previous studies, Mas presents different functional activities. In the present study, the functional mechanisms of… read more here.
Keywords: trypsin like; serine protease; expression; pcmas ... See more keywords
Coevolved Positions Represent Key Functional Properties in the Trypsin-Like Serine Proteases Protein Family
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DOI: 10.1021/acs.jcim.9b00903
Abstract: Trypsin-like Serine Proteases are a group of homologous enzymes which exert multiple roles in both vertebrate and invertebrate organisms. Key properties of these enzymes include their activation from an inactive zymogen form to their active… read more here.
Keywords: trypsin like; like serine; serine proteases; protein family ... See more keywords
Chemically diverse activity-based probes with unexpected inhibitory mechanisms targeting trypsin-like serine proteases
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Chemistry"
DOI: 10.3389/fchem.2022.1089959
Abstract: Activity-based probes (ABP) are molecules that bind covalently to the active form of an enzyme family, making them an attractive tool for target and biomarker identification and drug discovery. The present study describes the synthesis… read more here.
Keywords: like serine; based probes; activity based; trypsin like ... See more keywords