Chemo-Selection Strategy for Limited Proteolysis Experiments on the Proteomic Scale.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.8b04122
Abstract: Described here is a chemo-selective enrichment strategy, termed the semitryptic peptide enrichment strategy for proteolysis procedures (STEPP), to isolate the semitryptic peptides generated in mass spectrometry-based proteome-wide applications of limited proteolysis methods. The strategy involves… read more here.
Keywords: strategy; proteolysis; chemo; proteolysis experiments ... See more keywords
Measuring protein structural changes on a proteome-wide scale using limited proteolysis-coupled mass spectrometry
Sign Up to like & getrecommendations! Published in 2017 at "Nature Protocols"
DOI: 10.1038/nprot.2017.100
Abstract: Protein structural changes induced by external perturbations or internal cues can profoundly influence protein activity and thus modulate cellular physiology. A number of biophysical approaches are available to probe protein structural changes, but these are… read more here.
Keywords: structural changes; mass spectrometry; proteolysis coupled; protein structural ... See more keywords
Proteases Immobilization for In Situ Time-Limited Proteolysis on MALDI Chips
Sign Up to like & getrecommendations! Published in 2019 at "Catalysts"
DOI: 10.3390/catal9100833
Abstract: A large number of different enzyme immobilization techniques are used in the field of life sciences, clinical diagnostics, or biotechnology. Most of them are based on a chemically mediated formation of covalent bond between an… read more here.
Keywords: maldi chips; immobilization; situ time; limited proteolysis ... See more keywords