Impact of pre-amplification conditions on sensitivity of the tat/rev induced limiting dilution assay
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DOI: 10.1007/s00705-018-3894-7
Abstract: Antiretroviral therapy (ART) can lower a patient’s HIV plasma viral load to an undetectable level, but following cessation of ART viremia rapidly rebounds. It has been shown that ART does not eliminate latent viruses sequestered… read more here.
Keywords: tat rev; rev induced; limiting dilution; pre amplification ... See more keywords
Use of Limiting Dilution Method for Isolation of Nucleus Pulposus Mesenchymal Stem/Progenitor Cells and Effects of Plating Density on Biological Characteristics and Plasticity
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DOI: 10.1155/2017/9765843
Abstract: Objectives To evaluate the effects of the limiting dilution method and plating density in rat nucleus pulposus mesenchymal stem/progenitor cells (NPMSCs). Materials and Methods Nucleus pulposus tissues were isolated from 12-week-old male Sprague-Dawley rats and… read more here.
Keywords: dilution method; cell; limiting dilution; density ... See more keywords
Targeted Methylation Profiling of Single Laser-Capture Microdissected Post-Mortem Brain Cells by Adapted Limiting Dilution Bisulfite Pyrosequencing (LDBSP)
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DOI: 10.3390/ijms232415571
Abstract: A reoccurring issue in neuroepigenomic studies, especially in the context of neurodegenerative disease, is the use of (heterogeneous) bulk tissue, which generates noise during epigenetic profiling. A workable solution to this issue is to quantify… read more here.
Keywords: methylation; dilution bisulfite; methylation profiling; laser capture ... See more keywords