Heparin enables the reliable detection of endotoxin in human serum samples using the Limulus amebocyte lysate assay
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DOI: 10.1038/s41598-024-52735-8
Abstract: The determination of lipopolysaccharide (endotoxin) in serum or plasma samples using Limulus amebocyte lysate (LAL)-based assays is currently not sufficiently reliable in clinical diagnostics due to numerous interfering factors that strongly reduce the recovery of… read more here.
Keywords: samples using; heparin; serum; limulus amebocyte ... See more keywords
Limitations of Limulus amebocyte lysate test for endotoxin control in raw materials for liposomal nanoformulations.
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DOI: 10.1080/17435889.2024.2395243
Abstract: Aim: To evaluate the applicability of Limulus amebocyte lysate (LAL) assay for endotoxin determination in lipid compounding liposomal nanoformulations.Materials & methods: Spiked cholesterol, hydrogenated soy phosphatidylcholine and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000] (DSPE-PEG 2000) samples with endotoxins, simulating… read more here.
Keywords: endotoxin control; amebocyte lysate; control raw; liposomal nanoformulations ... See more keywords
Endotoxin detection in nanoliposomes using diluted Limulus Amebocyte Lysate and isothermal titration calorimetry
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DOI: 10.1515/pac-2025-0432
Abstract: Abstract The increasing use of nanomaterial-based products, such as nanoliposome vaccines, has amplified the need for reliable endotoxin testing, particularly for intravenous applications. Conventional Limulus Amebocyte Lysate (LAL) assays can be compromised by nanomaterials or… read more here.
Keywords: detection; amebocyte lysate; titration calorimetry; isothermal titration ... See more keywords