Linkage‐Specific Synthesis of Di‐ubiquitin Probes Enabled by the Incorporation of Unnatural Amino Acid ThzK
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DOI: 10.1002/cbic.202200133
Abstract: Di‐ubiquitin (diUB) conjugates of defined linkages are useful tools for probing the functions of UB ligases, UB‐binding proteins and deubiquitinating enzymes (DUBs) in coding, decoding and editing the signals carried by the UB chains. Here… read more here.
Keywords: incorporation unnatural; linkage; specific synthesis; unnatural amino ... See more keywords
Synthesis and purification of linkage-specific polyubiquitin chains of distinct length for structural studies.
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DOI: 10.1016/j.ab.2018.08.007
Abstract: Polyubiquitylation is one of the most versatile post-translational modifications involved in the regulation of numerous intracellular signaling processes. An assembly procedure that is simple, robust, and efficient to synthesize and purify linkage-specific polyubiquitin chains of… read more here.
Keywords: structural studies; linkage specific; polyubiquitin chains; specific polyubiquitin ... See more keywords
Quantitative LC-MS and MS/MS analysis of sialylated glycans modified by linkage-specific alkylamidation.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2018.11.014
Abstract: Sialic acids (Sia) are involved in various biological and pathological processes, and are often found attached to non-reducing ends of glycans through either α2,3- or α2,6-linkages. To quantitatively analyze glycan structures with these linkage isoforms… read more here.
Keywords: specific alkylamidation; analysis; method; linkage ... See more keywords