Preparation and assessment of cross-linked enzyme aggregates (CLEAs) of β-galactosidase from Lactobacillus leichmannii 313
Sign Up to like & getrecommendations! Published in 2020 at "Food and Bioproducts Processing"
DOI: 10.1016/j.fbp.2020.08.004
Abstract: Abstract β-galactosidases from Lactobacillus leichmannii 313 (LL 313) were immobilized for the first time by cross-linked enzyme aggregates (CLEA) technology. By using three precipitants (saturated ammonium sulfate (ASF), acetone (ACT), and isopropanol (IPA) in CLEA… read more here.
Keywords: enzyme aggregates; lactobacillus leichmannii; leichmannii 313; linked enzyme ... See more keywords
Development and characterization of cross-linked enzyme aggregates of thermotolerant alkaline protease from Bacillus licheniformis.
Sign Up to like & getrecommendations! Published in 2018 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2018.03.009
Abstract: An alkaline protease was produced by B. licheniformis with 132.43±3.4U/mL activity in LSF which was further enhanced by optimizing culture conditions. The optimum enzyme activity (148.9±4.1U/mL) was harvested at pH7.5; temperature, 40°C and inoculum, 1.5mL… read more here.
Keywords: enzyme aggregates; protease; linked enzyme; cross linked ... See more keywords
Cross-linked enzyme aggregates of alginate lyase: A systematic engineered approach to controlled degradation of alginate hydrogel.
Sign Up to like & getrecommendations! Published in 2018 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2018.03.110
Abstract: An enzyme aggregate of alginate lyase (EC 4.2.2.3) from flavobactierium was prepared using ammonium sulfate. The resultant aggregates upon cross-linking with glutaraldehyde produced insoluble and catalytically active cross-linked enzyme aggregate (CLEA) enzyme. The catalytic activity… read more here.
Keywords: alginate; alginate lyase; linked enzyme; cross linked ... See more keywords
Combining Pd nanoparticles on MOFs with cross-linked enzyme aggregates of lipase as powerful chemoenzymatic platform for one-pot dynamic kinetic resolution of amines
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Catalysis"
DOI: 10.1016/j.jcat.2019.08.033
Abstract: Abstract Acquisition of chiral amines is a very important item in scientific research. Chemoenzymatic catalyst combination of Pd nanoparticles supported on the external surface of the ethylenediamine-functionalized MIL-101 (Pd@ED-MIL-101) with layered cross-linked enzyme aggregates of… read more here.
Keywords: enzyme aggregates; pot dynamic; linked enzyme; pot ... See more keywords
Magnetic cross-linked enzyme aggregates (MCLEAs) applied to biomass conversion
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Solid State Chemistry"
DOI: 10.1016/j.jssc.2018.11.003
Abstract: Abstract The increasing energy and sustainable materials demand requires the development of renewable production technologies to meet current global demands. Certainly, the progress for a sustainable development goes through the use of renewable and versatile… read more here.
Keywords: magnetic cross; enzyme aggregates; conversion; biomass ... See more keywords
Activation of cellulase cross-linked enzyme aggregates (CLEAs) in scCO2
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Supercritical Fluids"
DOI: 10.1016/j.supflu.2019.104629
Abstract: Abstract The aim of this study was activation of cross-linked cellulase in supercritical carbon dioxide. Therefore at first, the process optimization for the preparation of cross-linked enzyme aggregates from enzyme cellulase using glutaraldehyde, was done.… read more here.
Keywords: enzyme aggregates; linked enzyme; cross linked; cross ... See more keywords
Co-Immobilizing Two Glycosidases Based on Cross-Linked Enzyme Aggregates to Enhance Enzymatic Properties for Achieving High Titer Icaritin Biosynthesis.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.2c04253
Abstract: Icaritin is a rare and high-value isopentane flavonoid compound with remarkable activities. Increasing yields while reducing cost has been a great challenge in icaritin production. Herein, we first reported a high titer icaritin biosynthesis strategy… read more here.
Keywords: cross linked; high titer; titer icaritin; linked enzyme ... See more keywords
Cross-linked enzyme aggregates immobilization: preparation, characterization, and applications
Sign Up to like & getrecommendations! Published in 2022 at "Critical Reviews in Biotechnology"
DOI: 10.1080/07388551.2022.2038073
Abstract: Abstract Enzymes are commonly used as biocatalysts for various biological and chemical processes. However, some major drawbacks of free enzymes (e.g. poor reusability and instability) significantly restrict their industrial practices. How to overcome these weaknesses… read more here.
Keywords: cross linked; enzyme aggregates; preparation; characterization ... See more keywords
Cross-linked enzyme-polymer conjugates with excellent stability and detergent-enhanced activity for efficient organophosphate degradation
Sign Up to like & getrecommendations! Published in 2018 at "Bioresources and Bioprocessing"
DOI: 10.1186/s40643-018-0236-2
Abstract: BackgroundEnzymatic biodegradation of organophosphate pesticides (OPs) is a promising technology to remove these toxic compounds. However, its application in industrial washing was restricted by the lack of efficient immobilized enzymes that can work at high… read more here.
Keywords: linked enzyme; cross linked; polymer; stability ... See more keywords
Tyrosinase Magnetic Cross-Linked Enzyme Aggregates: Biocatalytic Study in Deep Eutectic Solvent Aqueous Solutions
Sign Up to like & getrecommendations! Published in 2023 at "Biomolecules"
DOI: 10.3390/biom13040643
Abstract: In the field of biocatalysis, the implementation of sustainable processes such as enzyme immobilization or employment of environmentally friendly solvents, like Deep Eutectic Solvents (DESs) are of paramount importance. In this work, tyrosinase was extracted… read more here.
Keywords: deep eutectic; cross linked; enzyme aggregates; linked enzyme ... See more keywords
Preparation and Optimisation of Cross-Linked Enzyme Aggregates Using Native Isolate White Rot Fungi Trametes versicolor and Fomes fomentarius for the Decolourisation of Synthetic Dyes
Sign Up to like & getrecommendations! Published in 2017 at "International Journal of Environmental Research and Public Health"
DOI: 10.3390/ijerph15010023
Abstract: The key to obtaining an optimum performance of an enzyme is often a question of devising a suitable enzyme and optimisation of conditions for its immobilization. In this study, laccases from the native isolates of… read more here.
Keywords: linked enzyme; decolourisation; cross linked; fomes fomentarius ... See more keywords