Enrichment of N-Linked Glycopeptides and Their Identification by Complementary Fragmentation Techniques.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0528-8_17
Abstract: N-linked glycans are a ubiquitous posttranslational modification and are essential for correct protein folding in the endoplasmic reticulum of plants. However, this likely represents a narrow functional role for the diverse array of glycan structures currently… read more here.
Keywords: fragmentation techniques; enrichment linked; complementary fragmentation; linked glycopeptides ... See more keywords
Status Report on the High-Throughput Characterization of Complex Intact O-Glycopeptide Mixtures
Sign Up to like & getrecommendations! Published in 2018 at "Journal of The American Society for Mass Spectrometry"
DOI: 10.1007/s13361-018-1945-7
Abstract: AbstractA very complex mixture of intact, human N- and O-glycopeptides, enriched from the tryptic digest of urinary proteins of three healthy donors using a two-step lectin affinity enrichment, was analyzed by LC-MS/MS, leading to approximately… read more here.
Keywords: status report; throughput characterization; oxonium ions; linked glycopeptides ... See more keywords
l-cysteine-modified metal-organic frameworks as multifunctional probes for efficient identification of N-linked glycopeptides and phosphopeptides in human crystalline lens.
Sign Up to like & getrecommendations! Published in 2019 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2019.01.052
Abstract: Highly selective enrichment of N-linked glycopeptides and phosphopeptides from complex biological samples is extremely important prior to mass spectrometry analysis due to their low abundance as well as numerous extrinsic interferences. In this work, l-cysteine… read more here.
Keywords: cys; metal; human crystalline; glycopeptides phosphopeptides ... See more keywords
Glutathione-modified ordered mesoporous silicas for enrichment of N-linked glycopeptides by hydrophilic interaction chromatography.
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DOI: 10.1016/j.talanta.2020.121082
Abstract: Hydrophilic interaction chromatography (HILIC), as one of the important methods for glycopeptides enrichment, has attracted the attention of more and more researchers due to the high efficiency in glycopeptides enrichment. Ordered mesoporous silica materials have… read more here.
Keywords: mesoporous silicas; hydrophilic interaction; linked glycopeptides; interaction chromatography ... See more keywords
Data-Independent Acquisition-Based Mass Spectrometry (DIA-MS) for Quantitative Analysis of Intact N-Linked Glycopeptides.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.1c01659
Abstract: N-linked protein glycosylation is a key regulator in various biological functions. Previous studies have shown that aberrant glycosylation is associated with many diseases. Therefore, it is essential to elucidate protein modifications of glycosylation by quantitatively… read more here.
Keywords: analysis; analysis intact; intact glycopeptides; linked glycopeptides ... See more keywords