A class of low‐cost alternatives to kifunensine for increasing high mannose N‐linked glycosylation for monoclonal antibody production in Chinese hamster ovary cells
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology Progress"
DOI: 10.1002/btpr.3076
Abstract: N‐linked glycosylation of therapeutic monoclonal antibodies is an important product quality attribute for drug safety and efficacy. An increase in the percent of high mannose N‐linked glycosylation may be required for drug efficacy or to… read more here.
Keywords: high mannose; mannose linked; linked glycosylation; glycosylation ... See more keywords
N‐linked glycosylation of a subunit isoforms is critical for vertebrate vacuolar H+‐ATPase (V‐ATPase) biosynthesis
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Cellular Biochemistry"
DOI: 10.1002/jcb.26250
Abstract: The a subunit of the V0 membrane‐integrated sector of human V‐ATPase has four isoforms, a1‐a4, with diverse and crucial functions in health and disease. They are encoded by four conserved paralogous genes, and their vertebrate… read more here.
Keywords: atpase; linked glycosylation; subunit isoforms; subunit ... See more keywords
Predicted N‐terminal N‐linked glycosylation sites may underlie membrane protein expression patterns in Saccharomyces cerevisiae
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DOI: 10.1002/yea.3657
Abstract: N‐linked glycosylation is one type of posttranslational modification that proteins undergo during expression. The following describes the effects of N‐linked glycosylation on high‐level membrane protein expression in yeast with an emphasis on Saccharomyces cerevisiae. N‐linked… read more here.
Keywords: membrane protein; linked glycosylation; expression; glycosylation ... See more keywords
Effect of N-linked glycosylation on the activity and stability of a β-glucosidase from Putranjiva roxburghii.
Sign Up to like & getrecommendations! Published in 2018 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2018.01.201
Abstract: In this study, the effect of N-glycosylation on the conformational and functional stability of Putranjiva roxburghii family 1 β-glucosidase (PRGH1) enzyme was investigated. The deglycosylation of PRGH1 was carried out by using PNGase F enzyme… read more here.
Keywords: prgh1; effect; glycosylation; linked glycosylation ... See more keywords
N130, N175 and N207 are N-linked glycosylation sites of duck Tembusu virus NS1 that are important for viral multiplication, viremia and virulence in ducklings.
Sign Up to like & getrecommendations! Published in 2021 at "Veterinary microbiology"
DOI: 10.1016/j.vetmic.2021.109215
Abstract: Duck Tembusu virus (DTMUV) is an emerging mosquito-borne flavivirus that has caused acute egg-drop syndrome in egg-laying ducks. DTMUV nonstructural protein 1 (NS1) contains three potential predicted N-linked glycosylation sites at residues 130, 175 and… read more here.
Keywords: n130 n175; n175 n207; linked glycosylation; ns1 ... See more keywords
Glycan Residues Balance Analysis: A novel model for the N-linked glycosylation of IgG produced by CHO cells.
Sign Up to like & getrecommendations! Published in 2019 at "Metabolic engineering"
DOI: 10.1016/j.ymben.2019.08.016
Abstract: The structure of N-linked glycosylation is a very important quality attribute for therapeutic monoclonal antibodies. Different carbon sources in cell culture media, such as mannose and galactose, have been reported to have different influences on… read more here.
Keywords: mannose galactose; glycan residues; cho cells; model ... See more keywords
N-Linked Glycosylation Prevents Deamidation of Glycopeptide and Glycoprotein.
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DOI: 10.1021/acschembio.0c00734
Abstract: Deamidation has been recognized as a common spontaneous pathway of protein degradation and a prevalent concern in the pharmaceutical industry; deamidation caused the reduction of protein/peptide drug efficacy and shelf life in several cases. More… read more here.
Keywords: prevents deamidation; glycosylation prevents; glycosylation; linked glycosylation ... See more keywords
Glycosylation of viral proteins: Implication in virus–host interaction and virulence
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DOI: 10.1080/21505594.2022.2060464
Abstract: ABSTRACT Glycans are among the most important cell molecular components. However, given their structural diversity, their functions have not been fully explored. Glycosylation is a vital post-translational modification for various proteins. Many bacteria and viruses… read more here.
Keywords: viral proteins; glycosylation viral; linked glycosylation; glycosylation ... See more keywords
GALNTs: master regulators of metastasis-associated epithelial-mesenchymal transition (EMT)?
Sign Up to like & getrecommendations! Published in 2022 at "Glycobiology"
DOI: 10.1093/glycob/cwac014
Abstract: In humans, the UDP-N-α-D galactosamine:polypeptide N-acetylgalactosaminyltransferases family (ppGalNAc-Ts, GalNAc-Ts or GALNTs) comprises 20 isoenzymes. They are responsible for the initial synthesis of α-GalNAc1,3-O-Ser/Thr, or Tn antigen, at initiation of mucin type O-linked glycosylation. This structure… read more here.
Keywords: metastasis; mesenchymal transition; linked glycosylation; transition emt ... See more keywords
Oligosaccharyltransferase structures provide novel insight into the mechanism of asparagine-linked glycosylation in prokaryotic and eukaryotic cells.
Sign Up to like & getrecommendations! Published in 2019 at "Glycobiology"
DOI: 10.1093/glycob/cwy093
Abstract: Asparagine-linked (N-linked) glycosylation is one of the most common protein modification reactions in eukaryotic cells, occurring upon the majority of proteins that enter the secretory pathway. X-ray crystal structures of the single subunit OSTs from… read more here.
Keywords: mechanism; microscopy; linked glycosylation; glycosylation ... See more keywords
The consensus Nglyco -X-S/T motif and a previously unknown Nglyco -N -linked glycosylation are necessary for growth and pathogenicity of Phytophthora.
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DOI: 10.1111/1462-2920.15468
Abstract: Asparagine (Asn, N) -linked glycosylation within Nglyco -X-S/T; X ≠ P motif is a ubiquitously distributed post-translational modification that participates in diverse cellular processes. In this work, N-glycosylation inhibitor was shown to prevent Phytophthora sojae growth, suggesting… read more here.
Keywords: nglyco motif; glycosylation; glycosylation necessary; linked glycosylation ... See more keywords