Artificially Linked Ubiquitin Dimers Characterised Structurally and Dynamically by NMR Spectroscopy
Sign Up to like & getrecommendations! Published in 2019 at "Chembiochem"
DOI: 10.1002/cbic.201900146
Abstract: As one of the most prevalent post‐translational modifications in eukaryotic cells, ubiquitylation plays vital roles in many cellular processes, such as protein degradation, DNA metabolism, and cell differentiation. Substrate proteins can be tagged by distinct… read more here.
Keywords: artificially linked; dimers characterised; ubiquitin dimers; nmr spectroscopy ... See more keywords
Structural insights into K48-linked ubiquitin chain formation by the Pex4p-Pex22p complex.
Sign Up to like & getrecommendations! Published in 2018 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2017.12.150
Abstract: Pex4p is a peroxisomal E2 involved in ubiquitinating the conserved cysteine residue of the cycling receptor protein Pex5p. Previously, we demonstrated that Pex4p from the yeast Saccharomyces cerevisiae binds directly to the peroxisomal membrane protein… read more here.
Keywords: pex22p binding; k48 linked; pex4p; pex22p ... See more keywords
Molecular Recognition of M1-Linked Ubiquitin Chains by Native and Phosphorylated UBAN Domains.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of molecular biology"
DOI: 10.1016/j.jmb.2019.06.012
Abstract: Although the Ub-binding domain in ABIN proteins and NEMO (UBAN) is highly conserved, UBAN-containing proteins exhibit different Ub-binding properties, resulting in their diverse biological roles. Post-translational modifications further control UBAN domain specificity for poly-Ub chains.… read more here.
Keywords: uban domains; recognition linked; molecular recognition; linked chains ... See more keywords
Ufd2p synthesizes branched ubiquitin chains to promote the degradation of substrates modified with atypical chains
Sign Up to like & getrecommendations! Published in 2017 at "Nature Communications"
DOI: 10.1038/ncomms14274
Abstract: Ubiquitination of a subset of proteins by ubiquitin chain elongation factors (E4), represented by Ufd2p in Saccharomyces cerevisiae, is a pivotal regulator for many biological processes. However, the mechanism of Ufd2p-mediated ubiquitination is largely unclear.… read more here.
Keywords: substrates modified; degradation; ubiquitin chain; branched ubiquitin ... See more keywords
The retracted conformation of ubiquitin Ser65 phosphorylation inhibits the formation of K48-linked ubiquitin chains.
Sign Up to like & getrecommendations! Published in 2022 at "Bioscience reports"
DOI: 10.1042/bsr20220968
Abstract: PINK1, as the first reported ubiquitin kinase, can phosphorylate ubiquitin (Ub) at Ser65 site, which regulates the structure and function of Ub monomer. However, the levels of PINK1 and phosphorylated Ub (pUb) are very low… read more here.
Keywords: k48 linked; ubiquitin ser65; linked ubiquitin; formation k48 ... See more keywords
Mutational and Environmental Effects on the Dynamic Conformational Distributions of Lys48-Linked Ubiquitin Chains
Sign Up to like & getrecommendations! Published in 2023 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms24076075
Abstract: In multidomain proteins, individual domains connected by flexible linkers are dynamically rearranged upon ligand binding and sensing changes in environmental factors, such as pH and temperature. Here, we characterize dynamic domain rearrangements of Lys48-linked ubiquitin… read more here.
Keywords: lys48 linked; linked ubiquitin; conformational distributions; unit ... See more keywords