One pot synthesis of GDP‐mannose by a multi‐enzyme cascade for enzymatic assembly of lipid‐linked oligosaccharides
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DOI: 10.1002/bit.26454
Abstract: Glycosylation of proteins is a key function of the biosynthetic‐secretory pathway in the endoplasmic reticulum (ER) and Golgi apparatus. Glycosylated proteins play a crucial role in cell trafficking and signaling, cell‐cell adhesion, blood‐group antigenicity, and… read more here.
Keywords: cell; lipid linked; one pot; gdp ... See more keywords
Bacterial Cell Wall Modification with a Glycolipid Substrate.
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DOI: 10.1021/jacs.9b02290
Abstract: Despite the ubiquity and importance of glycans in biology, methods to probe their structures in cells are limited. Mammalian glycans can be modulated using metabolic incorporation, a process in which non-natural sugars are taken up… read more here.
Keywords: bacterial cell; wall; wall modification; lipid linked ... See more keywords
Visualizing conformation transitions of the Lipid II flippase MurJ
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DOI: 10.1038/s41467-019-09658-0
Abstract: The biosynthesis of many polysaccharides, including bacterial peptidoglycan and eukaryotic N-linked glycans, requires transport of lipid-linked oligosaccharide (LLO) precursors across the membrane by specialized flippases. MurJ is the flippase for the lipid-linked peptidoglycan precursor Lipid… read more here.
Keywords: transitions lipid; visualizing conformation; flippase; conformation transitions ... See more keywords
Reconstitution of the lipid-linked oligosaccharide pathway for assembly of high-mannose N-glycans
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DOI: 10.1038/s41467-019-09752-3
Abstract: The asparagine (N)-linked Man9GlcNAc2 is required for glycoprotein folding and secretion. Understanding how its structure contributes to these functions has been stymied by our inability to produce this glycan as a homogenous structure of sufficient… read more here.
Keywords: reconstitution lipid; pathway assembly; linked oligosaccharide; oligosaccharide pathway ... See more keywords