Development and validation of a modified TaqMan based real-time PCR assay targeting the lipl32 gene for detection of pathogenic Leptospira in canine urine samples
Sign Up to like & getrecommendations! Published in 2018 at "Brazilian Journal of Microbiology"
DOI: 10.1016/j.bjm.2017.09.004
Abstract: A modified TaqMan real-time polymerase chain reaction targeting a 138 bp fragment within the lipl32 gene was developed to identify exclusively pathogenic Leptospira spp. in dog urine samples. Thirty-five samples from dogs with suspected clinical… read more here.
Keywords: modified taqman; real time; pathogenic leptospira; gene ... See more keywords
Assessment of the immunogenicity of the leptospiral LipL32, LigAni, and LigBrep recombinant proteins in the sheep model.
Sign Up to like & getrecommendations! Published in 2019 at "Comparative immunology, microbiology and infectious diseases"
DOI: 10.1016/j.cimid.2019.05.012
Abstract: Veterinary leptospirosis vaccines are composed of bacterins and present limitations, for example, the need for bacteriological culture and serovar-dependent immunity. Recombinant antigens represent a promising alternative. LigAni, LigBrep, and LipL32 proteins have been shown to… read more here.
Keywords: lipl32; recombinant proteins; ligani ligbrep; group ... See more keywords
Leptospira collagenase and LipL32 for antibody detection in leptospirosis.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of immunological methods"
DOI: 10.1016/j.jim.2021.113168
Abstract: Various Leptospira components have been identified as candidate antigens for the detection of antibody to Leptospira. LipL32 is a Leptospira membrane protein which has been widely studied. The report of Leptospira whole-genome sequencing demonstrated that… read more here.
Keywords: detection; antibody detection; cola protein; cola ... See more keywords
Evaluation of LipL32 and LigA/LigB Knockdown Mutants in Leptospira interrogans Serovar Copenhageni: Impacts to Proteome and Virulence
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2021.799012
Abstract: Leptospirosis is a worldwide zoonosis caused by pathogenic species of the genus Leptospira. The recent application of CRISPR interference (CRISPRi) to Leptospira facilitates targeted gene silencing and provides a new tool to investigate pathogenic mechanisms… read more here.
Keywords: comparison control; lipl32; liga ligb; virulence ... See more keywords