CRISPR Interference and Activation to Modulate Transcription in Yarrowia lipolytica.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1414-3_6
Abstract: Recent developments in RNA-guided nuclease technologies have advanced the engineering of a wide range of organisms, including the nonconventional yeast Yarrowia lipolytica. Y. lipolytica has been the focus of a range of synthetic biology and… read more here.
Keywords: transcription; crispr interference; biology; activation ... See more keywords
Implementing CRISPR-Cas12a for Efficient Genome Editing in Yarrowia lipolytica.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1414-3_7
Abstract: The oleaginous yeast Yarrowia lipolytica has emerged as an industrially relevant chassis to produce various valuable chemicals. Metabolic engineering of Y. lipolytica relies on the availability of genetic engineering tools. Existing engineering strategies for this yeast… read more here.
Keywords: genome editing; crispr cas12a; efficient genome; lipolytica ... See more keywords
Advancing metabolic engineering of Yarrowia lipolytica using the CRISPR/Cas system
Sign Up to like & getrecommendations! Published in 2018 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-018-9366-x
Abstract: The oleaginous yeast Yarrowia lipolytica is widely used for the production of both bulk and fine chemicals, including organic acids, fatty acid-derived biofuels and chemicals, polyunsaturated fatty acids, single-cell proteins, terpenoids, and other valuable products.… read more here.
Keywords: metabolic engineering; lipolytica; engineering; crispr cas ... See more keywords
Enhanced itaconic acid production in Yarrowia lipolytica via heterologous expression of a mitochondrial transporter MTT
Sign Up to like & getrecommendations! Published in 2019 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-019-09627-z
Abstract: Itaconic acid, a promising platform chemical, has been applied in many fields of industrial production. As a potential candidate for itaconic acid production, Yarrowia lipolytica possesses several innate abilities such as the tolerance of low-pH… read more here.
Keywords: acid production; lipolytica; itaconic acid; acid ... See more keywords
Engineering Yarrowia lipolytica for arachidonic acid production through rapid assembly of metabolic pathway
Sign Up to like & getrecommendations! Published in 2017 at "Biochemical Engineering Journal"
DOI: 10.1016/j.bej.2016.12.004
Abstract: Abstract Yarrowia lipolytica, a non-conventional oleaginous yeast with special traits, has attracted increasing interest for producing value-added products. Generally, the DNA fragments of these heterologous metabolic pathways are constructed via the classic restriction digestion and… read more here.
Keywords: engineering; production; method; arachidonic acid ... See more keywords
Efficacy of Yarrowia lipolytica in the biocontrol of green mold and blue mold in Citrus reticulata and the mechanisms involved
Sign Up to like & getrecommendations! Published in 2019 at "Biological Control"
DOI: 10.1016/j.biocontrol.2019.104096
Abstract: Abstract Green mold and blue mold caused by Penicillium digitatum and P. italicum respectively, are two main fungal diseases in Citrus reticulata (mandarin oranges) worldwide. They are difficult to prevent for the lack of effective… read more here.
Keywords: green mold; blue mold; mold; mold blue ... See more keywords
Overexpression of GRAS Rhizomucor miehei Lipase in Yarrowia lipolytica via Optimizing Promoter, Gene Dosage and Fermentation Parameters.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of biotechnology"
DOI: 10.1016/j.jbiotec.2019.09.004
Abstract: Rhizomucor miehei lipase (RML), a GRAS catalyst with wide applications, was overexpressed in Yarrowia lipolytica, also a GRAS unconventional yeast, via a combined strategy, optimization for promoter, gene dosage and fermentation process. The lipase activity… read more here.
Keywords: rhizomucor miehei; lipolytica; rml; miehei lipase ... See more keywords
Effect of Yarrowia lipolytica RO25 cricket-based hydrolysates on sourdough quality parameters
Sign Up to like & getrecommendations! Published in 2021 at "Lwt - Food Science and Technology"
DOI: 10.1016/j.lwt.2021.111760
Abstract: Abstract The principal aim of this research was to use Yarrowia lipolytica RO25 to obtain a cricket powder-based hydrolysate to produce sourdough for bread production. RO25 hydrolysed cricket sourdough (RO25H-CS) was compared with a control… read more here.
Keywords: lipolytica ro25; lipolytica; yarrowia lipolytica; cricket ... See more keywords
Engineering of Yarrowia lipolytica for production of astaxanthin
Sign Up to like & getrecommendations! Published in 2017 at "Synthetic and Systems Biotechnology"
DOI: 10.1016/j.synbio.2017.10.002
Abstract: Astaxanthin is a red-colored carotenoid, used as food and feed additive. Astaxanthin is mainly produced by chemical synthesis, however, the process is expensive and synthetic astaxanthin is not approved for human consumption. In this study,… read more here.
Keywords: production astaxanthin; production; lipolytica production; lipolytica ... See more keywords
Exceptional solvent tolerance in Yarrowia lipolytica is enhanced by sterols.
Sign Up to like & getrecommendations! Published in 2019 at "Metabolic engineering"
DOI: 10.1016/j.ymben.2019.03.003
Abstract: Green organic solvents such as ionic liquids (ILs) have versatile use but are inhibitory to microbes even at low concentrations of 0.5-1.0% (v/v) ILs. We discovered the oleaginous yeast Yarrowia lipolytica can grow in 10%… read more here.
Keywords: tolerance; solvent tolerance; lipolytica; exceptional solvent ... See more keywords
Increased Cordycepin Production in Yarrowia lipolytica Using Combinatorial Metabolic Engineering Strategies.
Sign Up to like & getrecommendations! Published in 2023 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.2c00570
Abstract: As the first nucleoside antibiotic discovered in fungi, cordycepin, with its various biological activities, has wide applications. At present, cordycepin is mainly obtained from the natural fruiting bodies of Cordyceps militaris. However, due to long… read more here.
Keywords: metabolic engineering; engineering strategies; production; lipolytica ... See more keywords