Use of Peptide Arrays for Identification and Characterization of LIR Motifs.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8873-0_8
Abstract: The mammalian ATG8 proteins (LC3A-C/GABARAP, GABARAPL1, and GABARAPL2) are small ubiquitin-like proteins critically involved in macroautophagy. Their processed C-termini are posttranslationally conjugated to a phosphatidylethanolamine moiety, enabling their insertion into the lipid bilayers of both… read more here.
Keywords: lir; use peptide; lir motifs; arrays identification ... See more keywords
Monitoring LC3- or GABARAP-positive autophagic membranes using modified RavZ-based probes
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DOI: 10.1038/s41598-019-53372-2
Abstract: Xenophagy is a selective lysosomal degradation pathway for invading pathogens in host cells. However, invading bacteria also develop survival mechanisms to inhibit host autophagy. RavZ is a protein secreted by Legionella that irreversibly delipidates mammalian… read more here.
Keywords: lir; positive autophagic; ravz based; autophagic membranes ... See more keywords
An atypical LIR motif within UBA5 (ubiquitin like modifier activating enzyme 5) interacts with GABARAP proteins and mediates membrane localization of UBA5
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DOI: 10.1080/15548627.2019.1606637
Abstract: ABSTRACT Short linear motifs, known as LC3-interacting regions (LIRs), interact with mactoautophagy/autophagy modifiers (Atg8/LC3/GABARAP proteins) via a conserved universal mechanism. Typically, this includes the occupancy of 2 hydrophobic pockets on the surface of Atg8-family proteins… read more here.
Keywords: lir; ubiquitin like; interacts gabarap; gabarap proteins ... See more keywords