Evaluation of water‐assisted UV‐C light and its additive effect with peracetic acid for the inactivation of Listeria monocytogenes, Salmonella enterica and murine norovirus on whole and fresh‐cut strawberries during shelf‐life
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DOI: 10.1002/jsfa.11913
Abstract: Abstract BACKGROUND The purpose of the present study was to examine the inactivation of Salmonella enterica (50 μL; 109 CFU g−1), Listeria monocytogenes (50 μL; 109 CFU g−1), and murine norovirus (MNV‐1; 50 μL; 107 50% tissue culture infectious dose… read more here.
Keywords: water; murine norovirus; listeria monocytogenes; salmonella enterica ... See more keywords
Extraction and Analysis of Plasmid DNA from Listeria monocytogenes.
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DOI: 10.1007/978-1-0716-0982-8_12
Abstract: A plasmid preparation is a method used to extract and purify plasmid DNA. Methods developed to purify plasmid DNA from bacteria generally involve harvesting and alkaline lysis of the bacteria, precipitation of chromosomal DNA and… read more here.
Keywords: plasmid dna; extraction analysis; listeria monocytogenes; dna ... See more keywords
Mutant Construction and Integration Vector-Mediated Genetic Complementation in Listeria monocytogenes.
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DOI: 10.1007/978-1-0716-0982-8_14
Abstract: Genes that play a role in stress response mechanisms and other phenotypes of Listeria monocytogenes can be identified by construction and screening of mutant libraries. In this chapter, we describe the construction and screening of… read more here.
Keywords: construction; transposon; listeria monocytogenes; integration vector ... See more keywords
MALDI-ToF MS: A Rapid Methodology for Identifying and Subtyping Listeria monocytogenes.
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DOI: 10.1007/978-1-0716-0982-8_2
Abstract: Listeria monocytogenes is a major food-borne pathogen and causative agent of a fatal disease, listeriosis. Stringent regulatory guidelines and zero tolerance policy toward this bacterium necessitate rapid, accurate, and reliable methods of identification and subtyping.… read more here.
Keywords: methodology; maldi tof; tof rapid; identification ... See more keywords
Multilocus Sequence Typing (MLST) and Whole Genome Sequencing (WGS) of Listeria monocytogenes and Listeria innocua.
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DOI: 10.1007/978-1-0716-0982-8_7
Abstract: Nucleotide sequence-based methods focusing on the single-nucleotide polymorphisms (SNPs) of Listeria monocytogenes and L. innocua housekeeping genes (multilocus sequence typing) and in the core genome (core genome MLST) facilitate the rapid and interlaboratory comparison in… read more here.
Keywords: listeria; genome; sequence; sequence typing ... See more keywords
Systemic Listeria monocytogenes Infection as a Model to Study T Helper Cell Immune Responses.
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DOI: 10.1007/978-1-4939-9167-9_13
Abstract: Listeria monocytogenes, a Gram-positive facultative intracellular pathogen, has been widely used as a model for studying the immune response. Here, we describe a protocol for the systemic infection of mice with L. monocytogenes, followed by… read more here.
Keywords: infection; model; systemic listeria; cell ... See more keywords
Metabolic determinants in Listeria monocytogenes anaerobic listeriolysin O production
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DOI: 10.1007/s00203-017-1355-4
Abstract: Listeria monocytogenes is a human pathogen and a facultative anaerobe. To better understand how anaerobic growth affects L. monocytogenes pathogenesis, we first showed that anaerobic growth led to decreased growth and changes in surface morphology.… read more here.
Keywords: production; determinants listeria; metabolic determinants; monocytogenes anaerobic ... See more keywords
Menaquinone-mediated regulation of membrane fluidity is relevant for fitness of Listeria monocytogenes.
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DOI: 10.1007/s00203-021-02322-6
Abstract: Listeria monocytogenes is a food-borne pathogen with the ability to grow at low temperatures down to - 0.4 °C. Maintaining cytoplasmic membrane fluidity by changing the lipid membrane composition is important during growth at low temperatures. In… read more here.
Keywords: growth; membrane fluidity; membrane; listeria monocytogenes ... See more keywords
Molecular characterization and hematological analysis of Listeria monocytogenes infection in dairy cows in Punjab (Pakistan).
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DOI: 10.1007/s00203-022-02804-1
Abstract: Listeriosis is an emerging bacterial disease of animals and humans worldwide, caused by Listeria monocytogenes. The infected dairy cows continuously shed the microbes in their milk, a human being's concern. This study was designed to… read more here.
Keywords: analysis; milk; listeria; listeria monocytogenes ... See more keywords
Comparison of selected disinfectants efficiency against Listeria monocytogenes biofilm formed on various surfaces
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DOI: 10.1007/s10123-018-0002-5
Abstract: Listeria monocytogenes is a main etiological factor of listeriosis, spread mainly by food products. In recent years, an increasing number of patients with listeriosis and an augmentation in L. monocytogenes antibiotic resistance, e.g. to penicillin… read more here.
Keywords: comparison selected; biofilm formed; log cfu; listeria monocytogenes ... See more keywords
Green synthesis of salt-tolerant gold nanoparticles for the rapid qualitative detection of Listeria monocytogenes in lateral flow immunoassay
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DOI: 10.1007/s10853-020-05118-z
Abstract: Gold nanoparticles (AuNPs)-based lateral flow immunoassay (LFIA) is a widely used detection technique. Here, we developed a novel method for green synthesis of a salt-tolerant AuNPs by using aqueous extract of Damask rose petals (AEDR),… read more here.
Keywords: detection; flow immunoassay; gold nanoparticles; salt ... See more keywords