A Live‐Cell Epigenome Manipulation by Photo‐Stimuli‐Responsive Histone Methyltransferase Inhibitor
Sign Up to like & getrecommendations! Published in 2024 at "Advanced Science"
DOI: 10.1002/advs.202404608
Abstract: Post‐translational modifications on the histone H3 tail regulate chromatin structure, impact epigenetics, and hence the gene expressions. Current chemical modulation tools, such as unnatural amino acid incorporation, protein splicing, and sortase‐based editing, have allowed for… read more here.
Keywords: cell epigenome; photo; live cell; inhibitor ... See more keywords
BRIGHT Enables High-SNR Live-Cell Imaging of Non-Repetitive Sequences via Bivalent Fluorescent Nanobody-Mediated Cascade-Dependent Illumination.
Sign Up to like & getrecommendations! Published in 2025 at "Advanced science"
DOI: 10.1002/advs.202513014
Abstract: Genomic loci and genome‐independent DNA exhibit heterogeneous dynamics related to physiological function. Live‐cell imaging of non‐repetitive sequences is essential but limited by low signal‐to‐noise ratio (SNR), restricting accurate identification and dynamic tracking. Here, the development… read more here.
Keywords: non repetitive; live cell; dependent illumination; illumination ... See more keywords
Acidic pH‐Triggered Live‐Cell Lysosome Specific Tracking, Ratiometric pH Sensing, and Multicolor Imaging by Visible to NIR Switchable Cy‐7 Dyes
Sign Up to like & getrecommendations! Published in 2022 at "ChemBioChem"
DOI: 10.1002/cbic.202200641
Abstract: We have demonstrated an efficient synthetic route with crystal structures for the construction of acidic pH‐triggered visible‐to‐NIR interchangeable ratiometric fluorescent pH sensors. This bioresponsive probe exhibits pH‐sensitive reversible absorption/emission features, low cytotoxicity, a huge 322 nm… read more here.
Keywords: cell; visible nir; triggered live; live cell ... See more keywords
A guide for single-particle chromatin tracking in live cell nuclei.
Sign Up to like & getrecommendations! Published in 2022 at "Cell biology international"
DOI: 10.1002/cbin.11762
Abstract: The emergence of labeling strategies and live cell imaging methods enables the imaging of chromatin in living cells at single digit nanometer resolution as well as milliseconds temporal resolution. These technical breakthroughs revolutionize our understanding… read more here.
Keywords: chromatin; guide single; particle chromatin; single particle ... See more keywords
Multispectral Live‐Cell Imaging
Sign Up to like & getrecommendations! Published in 2018 at "Current Protocols in Cell Biology"
DOI: 10.1002/cpcb.46
Abstract: Fluorescent proteins and vital dyes are invaluable tools for studying dynamic processes within living cells. However, the ability to distinguish more than a few different fluorescent reporters in a single sample is limited by the… read more here.
Keywords: live cell; cell; cell imaging; multispectral live ... See more keywords
Photoactivatable Xanthone (PaX) Dyes Enable Quantitative, Dual Color, and Live‐Cell MINFLUX Nanoscopy
Sign Up to like & getrecommendations! Published in 2024 at "Small Methods"
DOI: 10.1002/smtd.202301497
Abstract: The single‐molecule localization concept MINFLUX has triggered a reevaluation of the features of fluorophores for attaining nanometer‐scale resolution. MINFLUX nanoscopy benefits from temporally controlled fluorescence (“on”/“off”) photoswitching. Combined with an irreversible switching behavior, the localization… read more here.
Keywords: live cell; minflux; minflux nanoscopy; dual color ... See more keywords
Live-Cell Imaging of Sirtuin Activity Using a One-Step Fluorescence Probe.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1258-3_14
Abstract: Sirtuins (SIRTs) are a family of NAD+-dependent histone deacetylases (HDACs). In mammals, dysfunction of SIRTs is associated with age-related metabolic diseases, cancers, and even aging. Therefore, the detection of SIRT activity in living cells or… read more here.
Keywords: fluorescence probe; sirt; activity; live cell ... See more keywords
Live-Cell Imaging of RNA Transport in Axons of Cultured Primary Neurons.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1990-2_11
Abstract: The use of fluorescent proteins has revolutionized the study of protein localization and transport. However, the visualization of other molecules and specifically RNA during live-cell imaging remains challenging. In this chapter, we provide guidance to… read more here.
Keywords: rna transport; live cell; transport; imaging rna ... See more keywords
Optimizing Long-Term Live Cell Imaging.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-2051-9_3
Abstract: Live cell microscopy has become a common technique for exploring dynamic biological processes. When combined with fluorescent markers of cellular structures of interest, or fluorescent reporters of a biological activity of interest, live cell microscopy… read more here.
Keywords: microscopy; optimizing long; long term; cell ... See more keywords
Live Cell Imaging Confocal Microscopy Analysis of HBV Myr-PreS1 Peptide Binding and Uptake in NTCP-GFP Expressing HepG2 Cells.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6700-1_3
Abstract: To obtain basic knowledge about specific molecular mechanisms involved in the entry of pathogens into cells is the basis for establishing pharmacologic substances blocking initial viral binding, infection, and subsequent viral spread. Lack of information… read more here.
Keywords: imaging confocal; cell imaging; live cell; microscopy ... See more keywords
Live-Cell STED Imaging with the HyPer2 Biosensor.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7265-4_3
Abstract: Stimulated emission depletion (STED) microscopy is a popular super resolution imaging technique. Not only synthetic dyes and fluorescent proteins can be utilized as STED fluorophores, but also genetically encoded biosensors. Fusing the biosensor with proteins… read more here.
Keywords: hyper2 biosensor; biosensor; cell sted; live cell ... See more keywords