Acidic pH‐Triggered Live‐Cell Lysosome Specific Tracking, Ratiometric pH Sensing, and Multicolor Imaging by Visible to NIR Switchable Cy‐7 Dyes
Sign Up to like & getrecommendations! Published in 2022 at "ChemBioChem"
DOI: 10.1002/cbic.202200641
Abstract: We have demonstrated an efficient synthetic route with crystal structures for the construction of acidic pH‐triggered visible‐to‐NIR interchangeable ratiometric fluorescent pH sensors. This bioresponsive probe exhibits pH‐sensitive reversible absorption/emission features, low cytotoxicity, a huge 322 nm… read more here.
Keywords: cell; visible nir; triggered live; live cell ... See more keywords
A guide for single-particle chromatin tracking in live cell nuclei.
Sign Up to like & getrecommendations! Published in 2022 at "Cell biology international"
DOI: 10.1002/cbin.11762
Abstract: The emergence of labeling strategies and live cell imaging methods enables the imaging of chromatin in living cells at single digit nanometer resolution as well as milliseconds temporal resolution. These technical breakthroughs revolutionize our understanding… read more here.
Keywords: chromatin; guide single; particle chromatin; single particle ... See more keywords
Multispectral Live‐Cell Imaging
Sign Up to like & getrecommendations! Published in 2018 at "Current Protocols in Cell Biology"
DOI: 10.1002/cpcb.46
Abstract: Fluorescent proteins and vital dyes are invaluable tools for studying dynamic processes within living cells. However, the ability to distinguish more than a few different fluorescent reporters in a single sample is limited by the… read more here.
Keywords: live cell; cell; cell imaging; multispectral live ... See more keywords
Live-Cell Imaging of Sirtuin Activity Using a One-Step Fluorescence Probe.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1258-3_14
Abstract: Sirtuins (SIRTs) are a family of NAD+-dependent histone deacetylases (HDACs). In mammals, dysfunction of SIRTs is associated with age-related metabolic diseases, cancers, and even aging. Therefore, the detection of SIRT activity in living cells or… read more here.
Keywords: fluorescence probe; sirt; activity; live cell ... See more keywords
Live-Cell Imaging of RNA Transport in Axons of Cultured Primary Neurons.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1990-2_11
Abstract: The use of fluorescent proteins has revolutionized the study of protein localization and transport. However, the visualization of other molecules and specifically RNA during live-cell imaging remains challenging. In this chapter, we provide guidance to… read more here.
Keywords: rna transport; live cell; transport; imaging rna ... See more keywords
Optimizing Long-Term Live Cell Imaging.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-2051-9_3
Abstract: Live cell microscopy has become a common technique for exploring dynamic biological processes. When combined with fluorescent markers of cellular structures of interest, or fluorescent reporters of a biological activity of interest, live cell microscopy… read more here.
Keywords: microscopy; optimizing long; long term; cell ... See more keywords
Live Cell Imaging Confocal Microscopy Analysis of HBV Myr-PreS1 Peptide Binding and Uptake in NTCP-GFP Expressing HepG2 Cells.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6700-1_3
Abstract: To obtain basic knowledge about specific molecular mechanisms involved in the entry of pathogens into cells is the basis for establishing pharmacologic substances blocking initial viral binding, infection, and subsequent viral spread. Lack of information… read more here.
Keywords: imaging confocal; cell imaging; live cell; microscopy ... See more keywords
Live-Cell STED Imaging with the HyPer2 Biosensor.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7265-4_3
Abstract: Stimulated emission depletion (STED) microscopy is a popular super resolution imaging technique. Not only synthetic dyes and fluorescent proteins can be utilized as STED fluorophores, but also genetically encoded biosensors. Fusing the biosensor with proteins… read more here.
Keywords: hyper2 biosensor; biosensor; cell sted; live cell ... See more keywords
Tracking GLUT2 Translocation by Live-Cell Imaging.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7507-5_18
Abstract: The facilitative glucose transporter (GLUT) family plays a key role in metabolic homeostasis, controlling the absorption rates and rapid response to changing carbohydrate levels. The facilitative GLUT2 transporter is uniquely expressed in metabolic epithelial cells… read more here.
Keywords: cell imaging; live cell; tracking glut2; translocation ... See more keywords
Live Cell Barcoding for Efficient Analysis of Small Samples by Mass Cytometry.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9454-0_9
Abstract: In mass cytometry, sample loss is of considerable concern due to the relative inefficiency of cell event collection compared to similar techniques such as flow cytometry. Cell stimulation and the harsh conditions required in the… read more here.
Keywords: mass cytometry; small samples; live cell; cytometry ... See more keywords
Live-Cell Imaging of Fluorescently Tagged Phloem Proteins with Confocal Microscopy.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9562-2_8
Abstract: Confocal laser scanning microscopy can enable observation of phloem cells in living tissues. Here we describe live imaging of phloem cells in the leaves and roots of Arabidopsis thaliana using fluorescently tagged proteins, either expressed… read more here.
Keywords: phloem; live cell; microscopy; phloem proteins ... See more keywords