A novel fluorescent endoplasmic reticulum marker for super‐resolution imaging in live cells
Sign Up to like & getrecommendations! Published in 2023 at "FEBS Letters"
DOI: 10.1002/1873-3468.14581
Abstract: Endoplasmic reticulum (ER) is a highly complicated and dynamic organelle that actively changes its shape and communicates with other organelles. Visualization of ER in live cells is of great importance to understand cellular activities. Here,… read more here.
Keywords: endoplasmic reticulum; marker super; super resolution; live cells ... See more keywords
Carbonate‐Based Fluorescent Chemical Tool for Uncovering Carboxylesterase 1 (CES1) Activity Variations in Live Cells
Sign Up to like & getrecommendations! Published in 2022 at "ChemBioChem"
DOI: 10.1002/cbic.202200069
Abstract: Carboxylesterase 1 (CES1) plays a key role in the metabolism of endogenous biomolecules and xenobiotics including a variety of pharmaceuticals. Despite the established importance of CES1 in drug metabolism, methods to study factors that can… read more here.
Keywords: carboxylesterase ces1; live cells; activity; ces1 ... See more keywords
Endogenous Proteins Modulation in Live Cells with Small Molecules and Light
Sign Up to like & getrecommendations! Published in 2022 at "ChemBioChem"
DOI: 10.1002/cbic.202200244
Abstract: Protein modulation by light illumination enables the investigation of biological function in high spatiotemporal precision. Compared to genetic methods, the small molecule approach is uniquely suited for modulating endogenous proteins. Endogenous protein modulation in live… read more here.
Keywords: molecules light; endogenous proteins; live cells; small molecules ... See more keywords
Harnessing Split‐Inteins as a Tool for the Selective Modification of Surface Receptors in Live Cells
Sign Up to like & getrecommendations! Published in 2022 at "ChemBioChem"
DOI: 10.1002/cbic.202200487
Abstract: Biochemical studies of integral membrane proteins are often hampered by low purification yields and technical limitations such as aggregation causing in vitro manipulations to be challenging. The ability of controlling proteins in live cells bypasses these… read more here.
Keywords: surface; live cells; inteins tool; split inteins ... See more keywords
Single-Particle Tracking and Trajectory Analysis of Fluorescent Nanodiamonds in Cell-Free Environment and Live Cells.
Sign Up to like & getrecommendations! Published in 2022 at "Small"
DOI: 10.1002/smll.202201395
Abstract: Diamond magnetometry can provide new insights on the production of free radicals inside live cells due to its high sensitivity and spatial resolution. However, the measurements often lack intracellular context for the recorded signal. In… read more here.
Keywords: trajectory analysis; live cells; single particle; analysis ... See more keywords
Assessment of ROS Production in the Mitochondria of Live Cells.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0896-8_2
Abstract: Production of reactive oxygen species (ROS) in the mitochondria plays multiple roles in physiology, and excessive production of ROS leads to the development of various pathologies. ROS in the mitochondria are generated by various enzymes,… read more here.
Keywords: production; mitochondria live; live cells; ros production ... See more keywords
Quantitative Determination and Imaging of Gαq Signaling in Live Cells via Split-Luciferase Complementation.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1258-3_7
Abstract: G Protein-coupled receptors (GPCRs) transduce signals elicited by bioactive chemical agents (ligands), such as hormones, neurotransmitters, or cytokines, across the cellular membrane. Upon ligand binding, the receptor undergoes structural rearrangements, which cause the activation of… read more here.
Keywords: split luciferase; live cells; activation signaling; luciferase complementation ... See more keywords
Imaging Translation Dynamics of Single mRNA Molecules in Live Cells.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7213-5_26
Abstract: mRNA translation is a key step in decoding the genetic information stored in DNA. Regulation of translation efficiency contributes to gene expression control and is therefore important for cell fate and function. Here, we describe… read more here.
Keywords: mrna molecules; translation dynamics; dynamics single; live cells ... See more keywords
Target-activated and ratiometric photochromic probe for “double-check” detection of toxic thiols in live cells
Sign Up to like & getrecommendations! Published in 2019 at "Science China Chemistry"
DOI: 10.1007/s11426-019-9490-x
Abstract: Photochromic molecules can achieve reversible isomerization upon alternate light irradiations, which offers a great opportunity to improve the precision of analytes detection and imaging in complicated biological environments. Previous reported photochromic probe exhibited only mono-color… read more here.
Keywords: detection; probe; live cells; toxic thiols ... See more keywords
Effects of radiofrequency field exposure on proteotoxic-induced and heat-induced HSF1 response in live cells using the bioluminescence resonance energy transfer technique
Sign Up to like & getrecommendations! Published in 2020 at "Cell Stress and Chaperones"
DOI: 10.1007/s12192-020-01172-3
Abstract: As of today, only acute effects of RF fields have been confirmed to represent a potential health hazard and they are attributed to non-specific heating (≥ 1 °C) under high-level exposure. Yet, the possibility that environmental RF… read more here.
Keywords: live cells; hsf1; exposure; induced hsf1 ... See more keywords
A split NanoLuc complementation-based human norovirus-like particle entry assay facilitates evaluation of anti-norovirus antibodies in live cells.
Sign Up to like & getrecommendations! Published in 2021 at "Antiviral research"
DOI: 10.1016/j.antiviral.2021.105231
Abstract: Human noroviruses (NoVs) are the most common cause of acute gastroenteritis worldwide. One major obstacle in developing NoV vaccines is the lack of robust cell culture for efficacy evaluation. In this study, we successfully developed… read more here.
Keywords: entry; like particle; entry assay; lgbit ... See more keywords