A novel fluorescent endoplasmic reticulum marker for super‐resolution imaging in live cells
Sign Up to like & getrecommendations! Published in 2023 at "FEBS Letters"
DOI: 10.1002/1873-3468.14581
Abstract: Endoplasmic reticulum (ER) is a highly complicated and dynamic organelle that actively changes its shape and communicates with other organelles. Visualization of ER in live cells is of great importance to understand cellular activities. Here,… read more here.
Keywords: endoplasmic reticulum; marker super; super resolution; live cells ... See more keywords
Tracking the Endosomal Escape of Nanoparticles in Live Cells Using a Triplex‐Forming Oligonucleotide
Sign Up to like & getrecommendations! Published in 2024 at "Advanced Functional Materials"
DOI: 10.1002/adfm.202311240
Abstract: Nanoparticle‐mediated intracellular delivery of oligonucleotides is a complex phenomenon that depends on the architecture and the intracellular trafficking of the engineered nanoparticles. Unravelling the molecular arrangements of oligonucleotides within the nanoparticles as well as their… read more here.
Keywords: live cells; escape nanoparticles; forming oligonucleotide; escape ... See more keywords
Carbonate‐Based Fluorescent Chemical Tool for Uncovering Carboxylesterase 1 (CES1) Activity Variations in Live Cells
Sign Up to like & getrecommendations! Published in 2022 at "ChemBioChem"
DOI: 10.1002/cbic.202200069
Abstract: Carboxylesterase 1 (CES1) plays a key role in the metabolism of endogenous biomolecules and xenobiotics including a variety of pharmaceuticals. Despite the established importance of CES1 in drug metabolism, methods to study factors that can… read more here.
Keywords: carboxylesterase ces1; live cells; activity; ces1 ... See more keywords
Endogenous Proteins Modulation in Live Cells with Small Molecules and Light
Sign Up to like & getrecommendations! Published in 2022 at "ChemBioChem"
DOI: 10.1002/cbic.202200244
Abstract: Protein modulation by light illumination enables the investigation of biological function in high spatiotemporal precision. Compared to genetic methods, the small molecule approach is uniquely suited for modulating endogenous proteins. Endogenous protein modulation in live… read more here.
Keywords: molecules light; endogenous proteins; live cells; small molecules ... See more keywords
Harnessing Split‐Inteins as a Tool for the Selective Modification of Surface Receptors in Live Cells
Sign Up to like & getrecommendations! Published in 2022 at "ChemBioChem"
DOI: 10.1002/cbic.202200487
Abstract: Biochemical studies of integral membrane proteins are often hampered by low purification yields and technical limitations such as aggregation causing in vitro manipulations to be challenging. The ability of controlling proteins in live cells bypasses these… read more here.
Keywords: surface; live cells; inteins tool; split inteins ... See more keywords
Development of a Highly Selective NanoBRET Probe to Assess MAGL Inhibition in Live Cells
Sign Up to like & getrecommendations! Published in 2024 at "ChemBioChem"
DOI: 10.1002/cbic.202400704
Abstract: Cell‐free enzymatic assays are highly useful tools in early compound profiling due to their robustness and scalability. However, their inadequacy to reflect the complexity of target engagement in a cellular environment may lead to a… read more here.
Keywords: assess magl; inhibition live; live cells; magl inhibition ... See more keywords
Single-Particle Tracking and Trajectory Analysis of Fluorescent Nanodiamonds in Cell-Free Environment and Live Cells.
Sign Up to like & getrecommendations! Published in 2022 at "Small"
DOI: 10.1002/smll.202201395
Abstract: Diamond magnetometry can provide new insights on the production of free radicals inside live cells due to its high sensitivity and spatial resolution. However, the measurements often lack intracellular context for the recorded signal. In… read more here.
Keywords: trajectory analysis; live cells; single particle; analysis ... See more keywords
Energy Transfer Between i-Motif DNA Encapsulated Silver Nanoclusters and Fluorescein Amidite Efficiently Visualizes the Redox State of Live Cells.
Sign Up to like & getrecommendations! Published in 2024 at "Small"
DOI: 10.1002/smll.202401629
Abstract: The redox regulation, maintaining a balance between oxidation and reduction in living cells, is vital for cellular homeostasis, intricate signaling networks, and appropriate responses to physiological and environmental cues. Here, a novel redox sensor, based… read more here.
Keywords: dna encapsulated; silver nanoclusters; encapsulated silver; fluorescein amidite ... See more keywords
Assessment of ROS Production in the Mitochondria of Live Cells.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0896-8_2
Abstract: Production of reactive oxygen species (ROS) in the mitochondria plays multiple roles in physiology, and excessive production of ROS leads to the development of various pathologies. ROS in the mitochondria are generated by various enzymes,… read more here.
Keywords: production; mitochondria live; live cells; ros production ... See more keywords
Quantitative Determination and Imaging of Gαq Signaling in Live Cells via Split-Luciferase Complementation.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1258-3_7
Abstract: G Protein-coupled receptors (GPCRs) transduce signals elicited by bioactive chemical agents (ligands), such as hormones, neurotransmitters, or cytokines, across the cellular membrane. Upon ligand binding, the receptor undergoes structural rearrangements, which cause the activation of… read more here.
Keywords: split luciferase; live cells; activation signaling; luciferase complementation ... See more keywords
Imaging Translation Dynamics of Single mRNA Molecules in Live Cells.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7213-5_26
Abstract: mRNA translation is a key step in decoding the genetic information stored in DNA. Regulation of translation efficiency contributes to gene expression control and is therefore important for cell fate and function. Here, we describe… read more here.
Keywords: mrna molecules; translation dynamics; dynamics single; live cells ... See more keywords