Streamlined duplex live‐dead microplate assay for cultured cells
Sign Up to like & getrecommendations! Published in 2017 at "Experimental Eye Research"
DOI: 10.1016/j.exer.2017.05.011
Abstract: ABSTRACT A duplex fluorescence assay to assess the viability of cells cultured in multi‐well plates is described, which can be carried out in the original culture plate using a plate reader, without exchanges of culture… read more here.
Keywords: live dead; cultured cells; fluorescence; assay ... See more keywords
Propidium ion enters viable cells with high membrane potential during live-dead staining.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of microbiological methods"
DOI: 10.1016/j.mimet.2017.09.011
Abstract: Live-dead staining with propidium iodide can give erroneous results for bacteria showing high membrane potentials. We observed uptake of propidium ions across intact cell membranes for Dinoroseobacter shibae and Bacillus subtilis. Apparently, a high membrane… read more here.
Keywords: live dead; high membrane; propidium; dead staining ... See more keywords
Phagocytosis of live and dead Escherichia coli and Staphylococcus aureus in human whole blood is markedly reduced by combined inhibition of C5aR1 and CD14.
Sign Up to like & getrecommendations! Published in 2019 at "Molecular immunology"
DOI: 10.1016/j.molimm.2019.03.014
Abstract: BACKGROUND Sepsis is a dysregulated host response to infection. The aim of this study was to investigate the effects of complement- and CD14 inhibition on phagocytosis of live and dead Gram-negative and Gram-positive bacteria in… read more here.
Keywords: live dead; phagocytosis; inhibition; coli ... See more keywords
Testing for human impacts in the mismatch of living and dead ostracode assemblages at nested spatial scales in subtropical lakes from the Bahamian archipelago
Sign Up to like & getrecommendations! Published in 2018 at "Paleobiology"
DOI: 10.1017/pab.2018.20
Abstract: Abstract Naturally time-averaged accumulations of skeletal remains—death assemblages—provide reliable, albeit temporally coarse, information on the species composition and structure of communities in diverse settings, and their mismatch with local living communities usually signals recent human-driven… read more here.
Keywords: live dead; dead agreement; ostracode assemblages; level ... See more keywords
Life span bias explains live–dead discordance in abundance of two common bivalves
Sign Up to like & getrecommendations! Published in 2018 at "Paleobiology"
DOI: 10.1017/pab.2018.32
Abstract: Abstract. Life span bias potentially alters species abundance in death assemblages through the overrepresentation of short-lived organisms compared with their long-lived counterparts. Although previous work found that life span bias did not contribute significantly to… read more here.
Keywords: live dead; discordance; life span; life ... See more keywords
Rose Bengal-Derived Ultrabright Sulfur-Doped Carbon Dots for Fast Discrimination between Live and Dead Cells.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.1c04658
Abstract: The discrimination between dead and live cells is crucial for cell viability evaluation. Carbon dots (CDs), with advantages like simple and cost-effective synthesis, excellent biocompatibility, and high photostability, have shown potential for realizing selective live/dead… read more here.
Keywords: cell; live dead; discrimination; dead cell ... See more keywords
A Carbon Nanotube Sensor Array for the Label-Free Discrimination of Live and Dead Cells with Machine Learning.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.1c04661
Abstract: Developing robust cell recognition strategies is important in biochemical research, but the lack of well-defined target molecules creates a bottleneck in some applications. In this paper, a carbon nanotube sensor array was constructed for the… read more here.
Keywords: label free; carbon nanotube; live dead; nanotube sensor ... See more keywords
Separating the Living from the Dead: An Electrophoretic Approach.
Sign Up to like & getrecommendations! Published in 2024 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.4c03336
Abstract: Cell viability studies are essential in numerous applications, including drug development, clinical analysis, bioanalytical assessments, food safety, and environmental monitoring. Microfluidic electrokinetic (EK) devices have been proven to be effective platforms to discriminate microorganisms by… read more here.
Keywords: live dead; separating living; dead coli; coli ... See more keywords
Propidium Monoazide is Unreliable for Quantitative Live–Dead Molecular Assays
Sign Up to like & getrecommendations! Published in 2025 at "Analytical Chemistry"
DOI: 10.1021/acs.analchem.4c05593
Abstract: Propidium monoazide (PMA) is a dye that distinguishes between live and dead cells in molecular assays like the Polymerase Chain Reaction (PCR). It works by cross-linking to the DNA of cells that have compromised membranes… read more here.
Keywords: pma; live dead; molecular assays; propidium monoazide ... See more keywords
New Diketopyrrolopyrrole-Based Ratiometric Fluorescent Probe for Intracellular Esterase Detection and Discrimination of Live and Dead Cells in Different Fluorescence Channels.
Sign Up to like & getrecommendations! Published in 2018 at "ACS applied materials & interfaces"
DOI: 10.1021/acsami.8b11365
Abstract: A new diketopyrrolopyrrole-based fluorescent probe (DPP-AM) was designed and synthesized for ratiometric detection of esterase and for imaging of live and dead cells in different modes. DPP-AM showed red fluorescence because of the intramolecular charge… read more here.
Keywords: live dead; detection; fluorescence; dpp ... See more keywords
Fluid Flow Induces Differential Detachment of Live and Dead Bacterial Cells from Nanostructured Surfaces
Sign Up to like & getrecommendations! Published in 2022 at "ACS Omega"
DOI: 10.1021/acsomega.2c01208
Abstract: Nanotopographic surfaces are proven to be successful in killing bacterial cells upon contact. This non-chemical bactericidal property has paved an alternative way of fighting bacterial colonization and associated problems, especially the issue of bacteria evolving… read more here.
Keywords: flow; nanotopographic surfaces; live dead; dead bacterial ... See more keywords