Live Imaging of Arabidopsis Axillary Meristems.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0183-9_7
Abstract: Axillary meristems (AMs) are established postembryonically at the leaf axils and can develop into lateral branches. The initiation of AMs establishes new growth axis and is of primary importance for understanding plant development. Understanding plant… read more here.
Keywords: imaging arabidopsis; arabidopsis axillary; biology; axillary meristems ... See more keywords
Live Imaging of Schwann Cell Development in Zebrafish.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7649-2_27
Abstract: The optical transparency of zebrafish larvae enables live imaging. Here we describe the methodology for live imaging and detail how to mount larvae for live imaging of Schwann cell development. read more here.
Keywords: imaging schwann; cell development; schwann cell; live imaging ... See more keywords
Live Imaging of mRNA Transcription in Drosophila Embryos.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8772-6_10
Abstract: Live imaging has been used in recent years for the understanding of dynamic processes in biology, such as embryo development. This was made possible by a combination of advancements in microscopy, leading to improved signal-to-noise… read more here.
Keywords: drosophila; mrna transcription; biology; imaging mrna ... See more keywords
Live Imaging of Cell Invasion Using a Multicellular Spheroid Model and Light-Sheet Microscopy.
Sign Up to like & getrecommendations! Published in 2017 at "Advances in experimental medicine and biology"
DOI: 10.1007/978-3-319-67358-5_11
Abstract: Three-dimensional cellular assays are becoming increasingly popular as a fundamental tool to bridge the gap between tissue culture systems and in vivo tissue. In particular, spheroids are recognised today as a necessary intermediate model between… read more here.
Keywords: imaging cell; microscopy; model; light sheet ... See more keywords
In vivo live imaging of bone cells
Sign Up to like & getrecommendations! Published in 2018 at "Histochemistry and Cell Biology"
DOI: 10.1007/s00418-018-1638-0
Abstract: There are as many as 200 cell types in the body, and highly sophisticated and varied life phenomena are carried out by cell migration to appropriate places at appropriate times following the appropriate interactions. Recent… read more here.
Keywords: vivo live; imaging bone; bone; bone cells ... See more keywords
HPM live μ for a full CLEM workflow.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in cell biology"
DOI: 10.1016/bs.mcb.2020.10.022
Abstract: With the development of advanced imaging methods that took place in the last decade, the spatial correlation of microscopic and spectroscopic information-known as multimodal imaging or correlative microscopy (CM)-has become a broadly applied technique to… read more here.
Keywords: fixation; clem; microscopy; hpm ... See more keywords
Live Imaging Reveals that the First Division of Differentiating Human Embryonic Stem Cells Often Yields Asymmetric Fates.
Sign Up to like & getrecommendations! Published in 2017 at "Cell reports"
DOI: 10.1016/j.celrep.2017.09.044
Abstract: How do stem cells respond to signals to initiate differentiation? Here, we show that, despite uniform exposure to differentiation-inducing extracellular signals, individual human embryonic stem cells (hESCs) respond heterogeneously. To track how hESCs incipiently exit… read more here.
Keywords: division; embryonic stem; stem; stem cells ... See more keywords
Live imaging using a FRET glucose sensor reveals glucose delivery to all cell types in the Drosophila brain.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of insect physiology"
DOI: 10.1016/j.jinsphys.2017.07.010
Abstract: All complex nervous systems are metabolically separated from circulation by a blood-brain barrier (BBB) that prevents uncontrolled leakage of solutes into the brain. Thus, all metabolites needed to sustain energy homeostasis must be transported across… read more here.
Keywords: cell; bbb; glucose sensor; brain ... See more keywords
Whole-plant live imaging of reactive oxygen species.
Sign Up to like & getrecommendations! Published in 2019 at "Molecular plant"
DOI: 10.1016/j.molp.2019.06.003
Abstract: Reactive oxygen species (ROS) are key regulators of numerous subcellular, cellular and systemic signals. They function in plants as an integral part of many different hormonal, physiological and developmental pathways, as well as play a… read more here.
Keywords: reactive oxygen; oxygen species; plant; whole plant ... See more keywords
Live imaging molecular changes in junctional tension upon VE-cadherin in zebrafish
Sign Up to like & getrecommendations! Published in 2017 at "Nature Communications"
DOI: 10.1038/s41467-017-01325-6
Abstract: Forces play diverse roles in vascular development, homeostasis and disease. VE-cadherin at endothelial cell-cell junctions links the contractile acto-myosin cytoskeletons of adjacent cells, serving as a tension-transducer. To explore tensile changes across VE-cadherin in live… read more here.
Keywords: cadherin tension; tension; cell; imaging molecular ... See more keywords
Long-term, in toto live imaging of cardiomyocyte behaviour during mouse ventricle chamber formation at single-cell resolution
Sign Up to like & getrecommendations! Published in 2020 at "Nature Cell Biology"
DOI: 10.1038/s41556-020-0475-2
Abstract: Mapping of the holistic cell behaviours sculpting the four-chambered mammalian heart has been a goal or previous studies, but so far only success in transparent invertebrates and lower vertebrates with two-chambered hearts has been achieved.… read more here.
Keywords: single cell; chamber formation; cell; ventricle chamber ... See more keywords