Purification processes of live virus vaccine candidates expressed in adherent Vero cell lines via multimodal chromatography in flowthrough mode.
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology and bioengineering"
DOI: 10.1002/bit.28430
Abstract: Live virus vaccine (LVV) purification, employing chromatography, can be challenged by low binding capacities and elution yields. Alternatively, processes relying solely on enzymatic digestion steps and size-based membrane separations can be limited by suboptimal reduction… read more here.
Keywords: purification; cell; live virus; chromatography ... See more keywords
Recombinant proteins of Zaire ebolavirus induce potent humoral and cellular immune responses and protect against live virus infection in mice.
Sign Up to like & getrecommendations! Published in 2018 at "Vaccine"
DOI: 10.1016/j.vaccine.2017.01.068
Abstract: Infections with filoviruses in humans are highly virulent, causing hemorrhagic fevers which result in up to 90% mortality. In addition to natural infections, the ability to use these viruses as bioterrorist weapons is of significant… read more here.
Keywords: immune; zaire ebolavirus; humoral cellular; immune responses ... See more keywords
Development of a broadly protective modified-live virus vaccine candidate against porcine reproductive and respiratory syndrome virus.
Sign Up to like & getrecommendations! Published in 2018 at "Vaccine"
DOI: 10.1016/j.vaccine.2017.11.028
Abstract: Modified-live virus (MLV) vaccines are widely used to protect pigs against porcine reproductive and respiratory syndrome virus (PRRSV). However, current MLV vaccines do not confer adequate levels of heterologous protection, presumably due to the substantial… read more here.
Keywords: con; modified live; vaccine; virus ... See more keywords
Comprehensive Comparison of Seven SARS-CoV-2-Specific Surrogate Virus Neutralization and Anti-Spike IgG Antibody Assays Using a Live-Virus Neutralization Assay as a Reference
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DOI: 10.1128/spectrum.02314-22
Abstract: While the measurement of neutralizing antibodies is considered a valuable tool in assessing protection against SARS-CoV-2, neutralization tests employ live-virus isolates and cell culture, requiring advanced laboratory biosafety levels. Including a large sample panel (over… read more here.
Keywords: neutralizing antibodies; live virus; neutralization; virus neutralization ... See more keywords
A Conserved Stem-Loop Structure within ORF5 Is a Frequent Recombination Hotspot for Porcine Reproductive and Respiratory Syndrome Virus 1 (PRRSV-1) with a Particular Modified Live Virus (MLV) Strain
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DOI: 10.3390/v15010258
Abstract: The emergence of recombinant PRRSV strains has been observed for more than a decade. These recombinant viruses are characterized by a genome that contains genetic material from at least two different parental strains. Due to… read more here.
Keywords: modified live; prrsv; stem loop; recombination ... See more keywords