Relative Quantification of siRNA Strand Loading into Ago2 for Design of Highly Active siRNAs.
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DOI: 10.1007/978-1-4939-9220-1_4
Abstract: In RNA interference (RNAi), silencing is achieved through the interaction of double-stranded small interfering RNAs (siRNAs) with essential RNAi pathway proteins, including Argonaute 2 (Ago2). Based on these interactions, one strand of the siRNA is… read more here.
Keywords: loading ago2; sirna strand; strand; relative quantification ... See more keywords
Analysis of AgoshRNA maturation and loading into Ago2
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DOI: 10.1371/journal.pone.0183269
Abstract: The RNA interference (RNAi) pathway was recently expanded by the discovery of multiple alternative pathways for processing of natural microRNA (miRNA) and man-made short hairpin RNA (shRNA) molecules. One non-canonical pathway bypasses Dicer cleavage and… read more here.
Keywords: loading ago2; agoshrna maturation; analysis agoshrna; agoshrna ... See more keywords