Single‐molecule localization microscopy goes quantitative
Sign Up to like & getrecommendations! Published in 2023 at "Microscopy Research and Technique"
DOI: 10.1002/jemt.24281
Abstract: In the last few years, single‐molecule localization (SMLM) techniques have been used to address biological questions in different research fields. More recently, super‐resolution has also been proposed as a quantitative tool for quantifying protein copy… read more here.
Keywords: localization microscopy; single molecule; molecule localization; microscopy ... See more keywords
Single Molecule Localization Microscopy for Studying Small Extracellular Vesicles.
Sign Up to like & getrecommendations! Published in 2023 at "Small"
DOI: 10.1002/smll.202205030
Abstract: Small extracellular vesicles (sEVs) are 30-200 nm nanovesicles enriched with unique cargoes of nucleic acids, lipids, and proteins. sEVs are released by all cell types and have emerged as a critical mediator of cell-to-cell communication. Although… read more here.
Keywords: localization microscopy; single molecule; extracellular vesicles; microscopy ... See more keywords
Super-resolution Analysis of TCR-Dependent Signaling: Single-Molecule Localization Microscopy.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6881-7_13
Abstract: Single-molecule localization microscopy (SMLM) comprises methods that produce super-resolution images from molecular locations of single molecules. These techniques mathematically determine the center of a diffraction-limited spot produced by a fluorescent molecule, which represents the most… read more here.
Keywords: molecule; super resolution; localization microscopy; microscopy ... See more keywords
Correlative 3D Structured Illumination Microscopy and Single-Molecule Localization Microscopy for Imaging Cancer Invasion.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7759-8_15
Abstract: Super-resolution microscopy methods enable resolution of biological molecules in their cellular or tissue context at the nanoscale. Different methods have their strengths and weaknesses. Here we present a method that enables correlative confocal, structured illumination… read more here.
Keywords: single molecule; microscopy; illumination microscopy; structured illumination ... See more keywords
Super-Resolution Imaging of Organelle Membrane Contact Sites via Far-Red Hyperspectral Localization Microscopy
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical Journal"
DOI: 10.1016/j.bpj.2016.11.780
Abstract: The communication and exchange of material between subcellular organelles is critical to many cellular processes. Areas of close apposition between organelles, termed membrane contact sites (MCS), have recently become a focus of study for intracellular… read more here.
Keywords: membrane contact; microscopy; hyperspectral localization; contact sites ... See more keywords
Optical diffraction tomography from single-molecule localization microscopy
Sign Up to like & getrecommendations! Published in 2021 at "Optics Communications"
DOI: 10.1016/j.optcom.2021.127290
Abstract: Abstract Single-molecule localization microscopy (SMLM) is a powerful method for the imaging of cellular structures. This modality delivers nanoscale resolution by sequentially activating a subset of fluorescent molecules and by extracting their super-resolved positions from the… read more here.
Keywords: localization microscopy; single molecule; microscopy; molecule localization ... See more keywords
Investigation of podosome ring protein arrangement using localization microscopy images.
Sign Up to like & getrecommendations! Published in 2017 at "Methods"
DOI: 10.1016/j.ymeth.2016.11.005
Abstract: Podosomes are adhesive structures formed on the plasma membrane abutting the extracellular matrix of macrophages, osteoclasts, and dendritic cells. They consist of an f-actin core and a ring structure composed of integrins and integrin-associated proteins.… read more here.
Keywords: microscopy; podosome ring; using localization; microscopy images ... See more keywords
About samples, giving examples: Optimized Single Molecule Localization Microscopy.
Sign Up to like & getrecommendations! Published in 2019 at "Methods"
DOI: 10.1016/j.ymeth.2019.05.008
Abstract: Super-resolution microscopy has profoundly transformed how we study the architecture of cells, revealing unknown structures and refining our view of cellular assemblies. Among the various techniques, the resolution of Single Molecule Localization Microscopy (SMLM) can… read more here.
Keywords: localization microscopy; single molecule; molecule localization; microscopy ... See more keywords
Single Molecule Localization Microscopy at Janelia's Advanced Imaging Center
Sign Up to like & getrecommendations! Published in 2020 at "Microscopy and Microanalysis"
DOI: 10.1017/s1431927620018619
Abstract: Single molecule localization microscopy (SMLM) was initially demonstrated nearly 15 years ago in a trio of seminal papers. Since then, it has been recognized an integral method among the broader superresolution imaging repertoire available to… read more here.
Keywords: localization microscopy; single molecule; microscopy; molecule localization ... See more keywords
Transcranial ultrafast ultrasound localization microscopy of brain vasculature in patients
Sign Up to like & getrecommendations! Published in 2021 at "Nature biomedical engineering"
DOI: 10.1038/s41551-021-00697-x
Abstract: Changes in cerebral blood flow are associated with stroke, aneurysms, vascular cognitive impairment, neurodegenerative diseases and other pathologies. Brain angiograms, typically performed via computed tomography or magnetic resonance imaging, are limited to millimetre-scale resolution and… read more here.
Keywords: microscopy; ultrafast ultrasound; localization microscopy; brain ... See more keywords
vLUME: 3D virtual reality for single-molecule localization microscopy
Sign Up to like & getrecommendations! Published in 2020 at "Nature Methods"
DOI: 10.1038/s41592-020-0962-1
Abstract: v LUME is a virtual reality software package designed to render large three-dimensional single-molecule localization microscopy datasets. v LUME features include visualization, segmentation, bespoke analysis of complex local geometries and exporting features. v LUME can… read more here.
Keywords: single molecule; microscopy; virtual reality; localization microscopy ... See more keywords