Locus‐Specific Detection of DNA Methylation: The Advance, Challenge, and Perspective of CRISPR‐Cas Assisted Biosensors
Sign Up to like & getrecommendations! Published in 2023 at "Small Methods"
DOI: 10.1002/smtd.202201624
Abstract: Deoxyribonucleic acid (DNA) methylation is one of the epigenetic characteristics that result in heritable and revisable phenotype changes but without sequence changes in DNA. Aberrant methylation occurring at a specific locus was reported to be… read more here.
Keywords: methylation; locus specific; dna methylation; detection ... See more keywords
Enzymatic Cleavage-Mediated Extension Stalling Enables Accurate Recognition and Quantification of Locus-Specific Uracil Modification in DNA.
Sign Up to like & getrecommendations! Published in 2023 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.3c01410
Abstract: Chemical modifications in DNA have profound influences on the structures and functions of DNA. Uracil, a naturally occurring DNA modification, can originate from the deamination of cytosine or arise from misincorporation of dUTP into DNA… read more here.
Keywords: modification; cleavage; quantification; dna ... See more keywords
Locus-specific chromatin isolation
Sign Up to like & getrecommendations! Published in 2020 at "Nature Reviews Molecular Cell Biology"
DOI: 10.1038/s41580-020-0217-0
Abstract: Identifying factors bound to specific genomic loci in an unbiased manner is the holy grail of chromatin research, because it would provide an ultimate description of locus function. In this Comment, we explain why purifying… read more here.
Keywords: chromatin isolation; chromatin; locus specific; specific chromatin ... See more keywords
Improved locus-specific unmethylated controls for MS-HRM analysis derived from 5-aza-2-deoxycytidine-treated DNA.
Sign Up to like & getrecommendations! Published in 2019 at "BioTechniques"
DOI: 10.2144/btn-2018-0161
Abstract: We report two restriction enzyme-based approaches for generating clean locus-specific unmethylated controls for methylation-sensitive high-resolution melting (MS-HRM) analyses. These unmethylated standards are derived from DNA treated with the demethylating agent 5-aza-2-deoxycytidine (5-Aza-dc). By using them, we… read more here.
Keywords: methylation; locus specific; unmethylated controls; treated dna ... See more keywords
Transcriptome Analysis of Human Endogenous Retroviruses at Locus-Specific Resolution in Non-Small Cell Lung Cancer
Sign Up to like & getrecommendations! Published in 2022 at "Cancers"
DOI: 10.3390/cancers14184433
Abstract: Simple Summary Lung cancer is the leading cause of cancer deaths worldwide. Most lung cancer patients are diagnosed with locally advanced or metastatic diseases, and their prognosis is relatively poor, with 5-year survival rates ranging… read more here.
Keywords: lung cancer; human endogenous; locus specific; lung ... See more keywords