Measuring Unzipping and Rezipping of Single Long DNA Molecules with Optical Tweezers.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8556-2_19
Abstract: The unwinding of double-stranded DNA is a frequently occurring event during the cellular processes of DNA replication, repair, and transcription. To help further investigate properties of this fundamental process as well as to study proteins… read more here.
Keywords: optical tweezers; dna; rezipping single; long dna ... See more keywords
Haplotype phasing of whole human genomes using bead-based barcode partitioning in a single tube
Sign Up to like & getrecommendations! Published in 2017 at "Nature Biotechnology"
DOI: 10.1038/nbt.3897
Abstract: Haplotype-resolved genome sequencing promises to unlock a wealth of information in population and medical genetics. However, for the vast majority of genomes sequenced to date, haplotypes have not been determined because of cumbersome haplotyping workflows… read more here.
Keywords: barcode partitioning; using bead; haplotype; long dna ... See more keywords
Sequence dependency of the thermodynamic properties of long DNA double-strands
Sign Up to like & getrecommendations! Published in 2017 at "RSC Advances"
DOI: 10.1039/c7ra05974d
Abstract: Using the Green’s function technique and tight-binding (TB) Hamiltonian method for π-electrons within a ladder model of long DNA double-strands acting as semiconducting nanowires, the temperature and sequence dependency of the Pauli paramagnetic susceptibility (PMS)… read more here.
Keywords: sequence dependency; long dna; dna double; double strands ... See more keywords
Replication-Dependent Organization Constrains Positioning of Long DNA Repeats in Bacterial Genomes
Sign Up to like & getrecommendations! Published in 2022 at "Genome Biology and Evolution"
DOI: 10.1093/gbe/evac102
Abstract: Bacterial genome organisation is primarily driven by chromosome replication from a single origin of replication. However chromosomal rearrangements, which can disrupt such organisation, are inevitable in nature. Long DNA repeats are major players mediating rearrangements,… read more here.
Keywords: dna; dna repeats; replication; bacterial genomes ... See more keywords
New algorithms for accurate and efficient de novo genome assembly from long DNA sequencing reads
Sign Up to like & getrecommendations! Published in 2022 at "Life Science Alliance"
DOI: 10.1101/2022.08.30.505891
Abstract: Innovative algorithmic approaches were used to perform assembly of complex genomes across the tree of life, from long DNA sequencing data. Building de novo genome assemblies for complex genomes is possible thanks to long-read DNA… read more here.
Keywords: new algorithms; sequencing reads; algorithms accurate; long dna ... See more keywords
Consistent ultra-long DNA sequencing with automated slow pipetting
Sign Up to like & getrecommendations! Published in 2021 at "BMC Genomics"
DOI: 10.1186/s12864-021-07500-w
Abstract: Background Oxford Nanopore Technologies’ instruments can sequence reads of great length. Long reads improve sequence assemblies by unambiguously spanning repetitive elements of the genome. Sequencing reads of significant length requires the preservation of long DNA… read more here.
Keywords: length; slow pipetting; library preparation; long dna ... See more keywords